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产品名称
N27 Rat Dopaminergic Neural Cell Line, The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.
生物来源
rat
质量水平
技术
cell culture | stem cell: suitable
运输
liquid nitrogen
1 of 4
此商品 | P6556 | SRE0047 | P8044 |
|---|---|---|---|
| specific activity ≥30 units/mg protein | specific activity ≥30 units/mg protein | specific activity ≥30 units/mg protein | specific activity ≥3.0 unit/mg solid |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| mol wt 28.93 kDa | mol wt 28.93 kDa | mol wt 28.93 kDa | mol wt 28.93 kDa |
一般描述
Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.
细胞系描述
Neural Stem Cells
Neural Lineage Cells
Neural Lineage Cells
应用
Research Category
Stem Cell Research
Neuroscience
Stem Cell Research
Neuroscience
Research Sub Category
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
质量
• Each vial contains ≥ 1X106 viable cells.
• Cells are negative for mycoplasma contamination.
• Cells are negative for mycoplasma contamination.
储存及稳定性
N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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Biochemical and biophysical research communications, 437, 380-385 (2013)
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Adams, F S, et al.
Neurochemical Research, 21, 619-627 (1996)
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Methods in molecular biology (Clifton, N.J.), 1996, 217-257 (2019-05-28)
Metabolomics has been successfully applied to study neurological and neurodegenerative disorders including Parkinson's disease for (1) the identification of potential biomarkers of onset and disease progression; (2) the identification of novel mechanisms of disease progression; and (3) the assessment of
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The Journal of biological chemistry, 294(25), 9973-9984 (2019-05-17)
A pathological feature of Parkinson's disease (PD) is Lewy bodies (LBs) composed of α-synuclein (α-syn) amyloid fibrils. α-Syn is a 140 amino acids-long protein, but truncated α-syn is enriched in LBs. The proteolytic processes that generate these truncations are not
Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
Prasad, K N, et al.
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