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一般描述
The chondrocyte is the only specialized cell type present in articular cartilage. They produce secretions to support and repair the cartilage matrix. Human chondrocytes in mature articular cartilage are post-mitotic and terminally differentiated cells. Studies on human chondrocytes have been hampered by the difficulty in obtaining sufficient numbers of primary human chondrocytes from a single joint and by variabilities among donors due to factors such as age and medical conditions.
T/C-28a2 cell line was established by transfecting primary cultures (day 5) of costal cartilage from a 15-year-old female with a retroviral vector expressing simian virus SV40 large T antigen.
References:
1. Goldring, M.B, Birkhead, J.R., Suen, L.F., Yamin, R., Mizuno, S., Glowacki, J., Arbiser, J.L, Apperley, J.F. (1994) Interleukin-1 beta-modulated gene expression in immortalized human chondrocytes. J. Clin. Invest 94(6): 2307-2316.
2. Claassen, H., Schicht, M., Brandt, J., Reuse, K., Schadlich, R., Goldring, M.B., Guddat, S.S., Thate, A., Paulsen, F. C-28/I2 and T/C-28a2 chondrocytes as well as human primary articular chondrocytes express hormone and insulin receptors- Useful cells in study of cartilage metabolism Ann Anat. 193(1): 23-29.
3. Goldring, M.B. (2004) Culture of immortalized chondrocytes and their use as models of chondrocyte function. Methods Mol. Med. 100: 37-52.
T/C-28a2 cell line was established by transfecting primary cultures (day 5) of costal cartilage from a 15-year-old female with a retroviral vector expressing simian virus SV40 large T antigen.
References:
1. Goldring, M.B, Birkhead, J.R., Suen, L.F., Yamin, R., Mizuno, S., Glowacki, J., Arbiser, J.L, Apperley, J.F. (1994) Interleukin-1 beta-modulated gene expression in immortalized human chondrocytes. J. Clin. Invest 94(6): 2307-2316.
2. Claassen, H., Schicht, M., Brandt, J., Reuse, K., Schadlich, R., Goldring, M.B., Guddat, S.S., Thate, A., Paulsen, F. C-28/I2 and T/C-28a2 chondrocytes as well as human primary articular chondrocytes express hormone and insulin receptors- Useful cells in study of cartilage metabolism Ann Anat. 193(1): 23-29.
3. Goldring, M.B. (2004) Culture of immortalized chondrocytes and their use as models of chondrocyte function. Methods Mol. Med. 100: 37-52.
细胞系描述
Chondrocyte Cells
应用
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
质量
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
储存及稳定性
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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