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安全信息

SCC034

Sigma-Aldrich

人间充质干细胞（骨髓）

Human mesenchymal stem cells derived from bone marrow that have a capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes.

别名:

Human Bone Marrow MSCs, Human Bone Marrow Multipotent Stem Cells, Human MSCs

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About This Item

UNSPSC代码:
41106509
eCl@ss:
32011203
NACRES:
NA.75

生物来源

human

质量水平

100

技术

cell culture | mammalian: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

输入

sample type mesenchymal stem cell(s)

运输

liquid nitrogen

一般描述

间充质干细胞，也称为骨髓基质细胞，被定义为具有粘附性骨髓来源的多能祖细胞的自我更新群体，具有分化为几种间充质细胞谱系的能力。 在体外确定的培养物中，间充质干细胞已显示易于分化为形成骨骼、软骨、脂肪、肌腱和肌肉组织的谱系特异性细胞。

从正常人骨髓中分离出EMD Millipore’s人间充质干细胞（骨髓），并在P2处冷冻保存，以确保最佳表型和最高活力和铺板效率。这些细胞作为贴壁细胞单层增殖，并显示出强大的体外扩增能力，同时保持其分化为多种谱系（包括脂肪细胞，成骨细胞和软骨细胞）的潜力。

细胞系描述

间充质干细胞

应用

研究类别
干细胞研究

组分

1x106活的人骨髓间充质干细胞

质量

-每个小瓶在P2处至少包含1x10⁶个活细胞。
-解冻时细胞活率大于75%，并且在初次解冻后可以将细胞培养至少3倍。
-骨髓供体的HIV、乙型肝炎和丙型肝炎检测为阴性。
-根据细胞遗传学分析，核型显然是正常的。
-细胞的支原体污染检测为阴性。

储存及稳定性

人间充质干细胞（骨髓）应保存在液氮中。保证细胞至少3次群体倍增，而不会显著影响细胞表面标志物的表达和分化潜能。

免责声明

仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品-Merck

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Adam R Wolfe et al.
Oncotarget, 7(50), 82482-82492 (2016-10-21)
Inflammatory breast cancer (IBC) is a unique and deadly disease with unknown drivers. We hypothesized the inflammatory environment contributes to the IBC phenotype. We used an in vitro co-culture system to investigate interactions between normal and polarized macrophages (RAW 264.7
Edo Dzafic et al.
BioMed research international, 2014, 508216-508216 (2014-04-12)
After removal of oocytes for in vitro fertilization, follicular aspirates which are rich in somatic follicular cells are discarded in daily medical practice. However, there is some evidence that less differentiated cells with stem cell characteristics are present among aspirated
Jung Eun Lee et al.
Polymers, 11(5) (2019-05-08)
Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to
Niall J Logan et al.
Bone, 117, 149-160 (2018-09-17)
Trauma-induced heterotopic ossification is an intriguing phenomenon involving the inappropriate ossification of soft tissues within the body such as the muscle and ligaments. This inappropriate formation of bone is highly prevalent in those affected by blast injuries. Here, we developed
Iriczalli Cruz-Maya et al.
Polymers, 12(8) (2020-07-31)
Electrospinning is the leading technology to fabricate fibrous scaffolds that mimic the architecture of the extracellular matrix of natural tissues. In order to improve the biological response, a consolidated trend involves the blending of synthetic polymers with natural proteins to
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实验方案

间充质干细胞培养方案

有关间充质的信息，特别是间充质干细胞培养实验方案用于间充质干细胞（MSC）分离、扩增和分化的分步细胞培养方案。

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Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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