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Merck
CN

SCC034

人间充质干细胞（骨髓）

Human mesenchymal stem cells derived from bone marrow that have a capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes.

别名:

Human Bone Marrow MSCs, Human Bone Marrow Multipotent Stem Cells, Human MSCs

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UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203

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产品名称

人间充质干细胞（骨髓）, Human mesenchymal stem cells derived from bone marrow that have a capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes.

biological source

human

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

input

sample type mesenchymal stem cell(s)

shipped in

liquid nitrogen

Quality Level

100

相关类别

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

Analysis Note

-每个小瓶在P2处至少包含1x10⁶个活细胞。
-解冻时细胞活率大于75%，并且在初次解冻后可以将细胞培养至少3倍。
-骨髓供体的HIV、乙型肝炎和丙型肝炎检测为阴性。
-根据细胞遗传学分析，核型显然是正常的。
-细胞的支原体污染检测为阴性。

Application

研究类别
干细胞研究

Biochem/physiol Actions

间充质干细胞

General description

间充质干细胞，也称为骨髓基质细胞，被定义为具有粘附性骨髓来源的多能祖细胞的自我更新群体，具有分化为几种间充质细胞谱系的能力。 在体外确定的培养物中，间充质干细胞已显示易于分化为形成骨骼、软骨、脂肪、肌腱和肌肉组织的谱系特异性细胞。

从正常人骨髓中分离出EMD Millipore’s人间充质干细胞（骨髓），并在P2处冷冻保存，以确保最佳表型和最高活力和铺板效率。这些细胞作为贴壁细胞单层增殖，并显示出强大的体外扩增能力，同时保持其分化为多种谱系（包括脂肪细胞，成骨细胞和软骨细胞）的潜力。

Other Notes

1x106活的人骨髓间充质干细胞

Preparation Note

人间充质干细胞（骨髓）应保存在液氮中。保证细胞至少3次群体倍增，而不会显著影响细胞表面标志物的表达和分化潜能。

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

高风险级别生物产品-Merck
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LncRNA-NEF is downregulated in postmenopausal osteoporosis and is related to course of treatment and recurrence.
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The Journal of international medical research, 47(7), 3299-3306 (2019-06-22)
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Critical limb ischemia (CLI) is the most severe form of peripheral artery disease and exhibits a high risk of lower extremity amputations. As even the most promising experimental approaches based on mesenchymal stem cells (MSCs) demonstrated only moderate therapeutic effects
Diego Omar Sanchez Ramirez et al.
Bioengineering (Basel, Switzerland), 8(12) (2021-12-24)
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Molecules (Basel, Switzerland), 27(7) (2022-04-13)
Poly-L-lactic acid (PLLA) aerogel-based scaffolds were obtained from physical PLLA gels containing cyclopentanone (CPO) or methyl benzoate (BzOMe) molecules. An innovative single step method of solvent extraction, using supercritical CO2, was used to achieve cylindrical monolithic aerogels. The pore distribution
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Journal of functional biomaterials, 14(1) (2023-01-21)
In the last decade, alginate-based microgels have gained relevant interest as three-dimensional analogues of extracellular matrix, being able to support cell growth and functions. In this study, core-shell microgels were fabricated by self-polymerization of dopamine (DA) molecules under mild oxidation
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商品

Mesenchymal Stem Cell Culture

Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

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实验方案

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

间充质干细胞培养方案

有关间充质的信息，特别是间充质干细胞培养实验方案用于间充质干细胞（MSC）分离、扩增和分化的分步细胞培养方案。

相关内容

A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells

We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.

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