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S7705

Sigma-Aldrich

TRAPeze1X CHAPS裂解缓冲液

1X CHAPS Lysis Buffer is a component of the gel-and ELISA-based TRAPeze Telomerase Detection Kits & is used in the extraction step of the procedure.

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UNSPSC代码:
41116012
eCl@ss:
32160405
NACRES:
NA.32

质量水平

制造商/商品名称

Chemicon®

运输

wet ice

一般描述

1X CHAPS 裂解缓冲液是基于凝胶和 ELISA 的 TRAPeze 端粒酶检测试剂盒的组件,用于该过程的提取步骤。
用作 TRAPEZE 试剂盒检测法的补充试剂,当较大组织样品需要使用额外的 1X CHAPS 裂解缓冲液才能实现有效的细胞裂解即可使用该试剂。 它通过使该试剂盒能分析较大的组织样本,从而扩展了试剂盒的功能。

应用

TRAPeze1X CHAPS裂解缓冲液已用于:
  • 用端粒酶定量活性测定(Q-TRAP)提取HEK293细胞总蛋白
  • 搅匀小鼠心房组织以进行蛋白质印迹分析
  • 在端粒酶表达分析细胞系中重悬细胞以确定蛋白浓度

储存及稳定性

储存于 4°C。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TRAPEZE is a trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Manuel Bernabé-García et al.
Molecular oncology, 15(7), 1818-1834 (2021-03-14)
Telomerase reverse transcriptase (TERT) maintains telomere homeostasis, thus ensuring chromosome stability and cell proliferation. In addition, several telomere-independent functions of human TERT have been described. In this study, we report that TERT binds directly to the TCF binding elements located

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