描述
The analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), aspartate transaminase 1 (GOT1), α-glutathianone S-transferase (GSTα), sorbitol dehydrogenase (SDH), and 5′-Nucleotidase (5′-NT/CD73).
质量水平
种属反应性
rat
制造商/商品名称
Milliplex®
assay range
accuracy: 77-92%
sensitivity: 0.021-4.038 ng/mL
(MinDC+2SD)
standard curve range: 0.02-15.0 ng/mL
(GSTα)
standard curve range: 0.04-30.0 ng/mL
(5′-NT/CD73)
standard curve range: 0.08-60.0 ng/mL
(GOT1)
standard curve range: 0.10-75.0 ng/mL
(ARG1)
standard curve range: 0.62-450.0 ng/mL
(SDH)
技术
multiplexing: suitable
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
一般描述
*ARG1, GSTα, and SDH are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.
The MILLIPLEX® portfolio provides a valuable research assay to investigate multiple biomarkers of liver injury in rat serum and plasma samples using the Luminex® xMAP® instrument platform. This kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum or plasma samples in duplicate.
Drug toxicity is the leading cause of acute liver failure in the United States. Patients with liver damage generally display elevated amounts of specific liver proteins in serum; these proteins can serve as biomarkers of drug-related liver toxicity. Monitoring these biomarkers can greatly help clinicians avoid drug-induced liver failure. Performing laboratory tests to characterize the side effects of potential therapeutics is an essential part of drug development. The rat is a leading animal model for these pre-clinical toxicity studies. The search for sensitive, organ-specific toxicity biomarkers is complemented by the development of novel assays to measure these critical analytes. Liver-Type Arginase 1 (ARG1), α-glutathione S-transferase (GSTα), and Sorbitol Dehydrogenase (SDH) are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. Aspartate transaminase 1 (GOT1) and 5′-Nucleotidase / CD73 (5′-NT) are traditional biomarkers recognized by both the Food and Drug Administration (FDA) and its European counterpart, the European Medicines Agency (EMA).
Panel Type: Toxicity
应用
- Analytes: 5′-Nucleotidase (5′-NT/CD73), Liver-Type Arginase 1 (ARG1), Aspartate transaminase 1 (GOT1), α-Glutathione S-Transferase (GSTα), Sorbitol Dehydrogenase (SDH)
- Recommended Sample Type: Rat serum and plasma
- Recommended Sample Dilution: 25 μL per well of 1:25 diluted serum or plasma
- Assay Run Time: 2 hours at room temperature (20-25°C)
- Research Category: Toxicity
特点和优势
组分
Rat Liver Injury Quality Control 1, 1 vial, lyophilized
Rat Liver Injury Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Liver Injury Detection Antibodies, 1 bottle, 3.2 mL
Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
其他说明
法律信息
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
相关内容
了解多重检测试剂盒——如基于Luminex®微珠多重免疫检测的MILLIPLEX®大鼠肝损伤Panel——如何帮助研究人员同时测量多种肝脏毒性生物标志物和细胞因子，加深对药物性肝损伤过程的认识。
Multiplex assays for hepatotoxic biomarker measurement in drug-induced liver injury research.
Multiplex toxicity assays enable researchers to simultaneously measure multiple toxicity biomarkers of various organs to gain a better understanding of toxicity. Explore how MILLIPLEX® multiplex toxicity assays are advancing research on liver injury, kidney toxicity, genotoxicity, and more.
了解在实验室、伴生和农业动物研究中使用生物标志物多重免疫检测产品的优势，探索MILLIPLEX®多重检测试剂盒在兽医学和动物健康研究方面的应用。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门