推荐产品
种属反应性
rat
质量水平
制造商/商品名称
Milliplex®
assay range
accuracy: 94-119%
sensitivity: 0.3-17 pg/mL
(MinDC)
standard curve range: 0.7-3,000 pg/mL
(IL-1β, TNFα)
standard curve range: 12-50,000 pg/mL
(PAI-1)
standard curve range: 15-60,000 pg/mL
(IL-6, Leptin)
standard curve range: 24-100,000 pg/mL
(Insulin)
standard curve range: 9.8-40,000 pg/mL
(MCP-1)
技术
multiplexing: suitable
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
一般描述
In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.
MILLIPLEX® Rat Adipokine Magnetic Bead Panel is a seven-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
MILLIPLEX® Rat Adipokine Magnetic Bead Panel is a seven-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
The MILLIPLEX® MAP Rat Adipokine Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 Total, and TNFα.
特异性
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
应用
- Analytes: IL-1β, IL-6, Insulin, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
- Recommended Sample Type: Rat serum or plasma
- Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
- Assay Run Time: Overnight (16-18) hours at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Endocrine; Obesity; Metabolic Disease
特点和优势
Design your multiplex kit by choosing available analytes within this panel.
包装
Everything you need in a single kit.
组分
Rat Adipokine Standard, 1 vial, lyophilized
Rat Adipokine Quality Control 1, 1 vial, lyophilized
Rat Adipokine Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Bead Diluent, 1 vial, 3.5 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Adipokine Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Rat Adipokine Quality Control 1, 1 vial, lyophilized
Rat Adipokine Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Bead Diluent, 1 vial, 3.5 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Adipokine Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
储存及稳定性
Recommended storage for kit components is 2 - 8°C.
其他说明
Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
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Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
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