用途
sufficient for 100 tests
质量水平
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
技术
immunoblotting: suitable
输入
sample type cell extract(s)
储存温度
−20°C
一般描述
检测试剂盒提供了独特的试剂组合，可用于从细胞质中分离出高度富集的线粒体部分。通过使用试剂盒随附的细胞色素 c 抗体进行免疫印迹，监测细胞色素 c 从线粒体部分到细胞质的转运。
组分
线粒体提取缓冲液、胞质溶胶提取缓冲液、蛋白酶抑制剂混合物、还原试剂、细胞色素 c 抗体和说明书。
警告
毒性：多个毒性值，请参阅 MSDS（O）
产品规格
试验时间：4 h
原理
细胞色素 c 释放细胞凋亡检测试剂盒提供了独特的试剂组合，可用于从细胞质中分离出高度富集的线粒体部分，可用于通过蛋白质印迹法检测细胞色素 c 。
制备说明
• 1X 胞质溶胶提取缓冲液：通过将 20 ml 5X 胞质溶胶提取缓冲液添加到 80 ml diH2O 中来制备 1X 胞质溶胶提取缓冲液；混合均匀。•线粒体提取缓冲液混合物： 在进行检测之前，为要检测的样品数量准备足够的线粒体提取缓冲液混合物。每个样品需要 0.1 ml 的混合物。要制备 1 ml 线粒体提取缓冲液混合物，请在 1 ml 线粒体提取缓冲液中加入 2 µl 500X 蛋白酶抑制剂混合物和 1 µl DTT；混合均匀。•胞质溶胶提取缓冲液混合物： 在进行检测之前，为要检测的样品数量准备足够的胞质溶胶缓冲液混合物。每个样品需要 1ml 混合物。要制备 1 ml 的胞质溶胶提取缓冲液混合物，请在 1 ml 胞质溶胶提取缓冲液中加入 2 µl 500X 蛋白酶抑制剂混合物和 1 µl DTT；混合均匀。
储存及稳定性
自收到之日起，将试剂盒的所有组件储存于 -20°C。打开后，将线粒体提取缓冲液和胞质溶胶提取缓冲液储存于 4°C，其余组件储存于 -20°C。
其他说明
由于该运输中有害物质的性质，您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系，以获取有关这些费用的更多信息。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
8A - Combustible corrosive hazardous materials
法规信息
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