用途
sufficient for 50 tests
质量水平
种属反应性（根据同源性预测）
all
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
技术
flow cytometry: suitable
输入
sample type cell suspension(s)
sample type frozen section(s)
sample type paraffin section(s)
sample type fixed-cell preparation(s)
检测方法
fluorometric
运输
wet ice
储存温度
−20°C
一般描述
可在单个细胞水平实现细胞凋亡的检测。易于检测凋亡形态。通过流式细胞术或荧光显微镜进行检测。试剂盒固封剂可观察总细胞数量。
货号QIA39是我们已被广泛引用的荧光素偶联版本的TdT Colorimetric FragEL DNA片段检测试剂盒的（货号QIA33），可用于非同位素标记。末端脱氧核苷酸转移酶（TdT）可结合响应凋亡信号而产生的DNA片段的暴露3′-OH末端，并催化生物素标记的和未标记的脱氧核苷酸加成。当激发时，荧光素产生强烈的信号，该信号可以通过荧光显微镜或流式细胞仪进行检测。固封剂可维持来自载玻片上标记样品的荧光信号，因此有助于正常细胞和凋亡细胞的形态评估和表征。非凋亡细胞可以通过DAPI滤镜进行观察。
组分
蛋白酶K、平衡和标记缓冲液、TdT酶（货号PF060）、对照载玻片、固封剂（货号HC08）和实验方案。
警告
毒性：多个毒性值，参见MSDS（O）
产品规格
试验时间：2 h
原理
Calbiochem Fluorescein-FragEL DNA碎片检测试剂盒是一种非同位素系统，可用于标记石蜡包埋的组织切片、组织冷冻切片、固定在载玻片上的细胞制剂、或细胞悬液中凋亡核中的DNA断裂。标记的检测和分析可以通过流式细胞仪或荧光显微镜进行。
储存及稳定性
荧光素-FragEL 试剂盒组分在干冰上进行运输。收到后，将试剂盒存放在-20°C非防冻冰箱中。为避免试剂损失在管盖中，请短暂离心所有解冻后的溶液，再取下管盖。（请参见下面的一般注意事项）。
其他说明
Darzynkiewicz, Z., et al. 1997.Cytometry27, 1.
Frey, T. 1997.Cytometry28, 253.
Shapiro, H.M.1995.Practical Flow Cytometry, Third Edition.Wiley-Liss Inc., New York, New York.
Darzynkiewicz, Z., et al. 1992.Cytometry13, 795.
Gavrieli, Y., et al. 1992.J. Cell Biol.119, 493.
Fawthrop, D. J., et al. 1991.Arch.Toxicol.65, 437.
Martin, S.J., et al. 1990.J. Immunology145, 1859.
Wyllie, A. H. 1980.Nature284, 555.
Kerr, J. F. R., et al. 1972.Br. J. Cancer26, 239.
Frey, T. 1997.Cytometry28, 253.
Shapiro, H.M.1995.Practical Flow Cytometry, Third Edition.Wiley-Liss Inc., New York, New York.
Darzynkiewicz, Z., et al. 1992.Cytometry13, 795.
Gavrieli, Y., et al. 1992.J. Cell Biol.119, 493.
Fawthrop, D. J., et al. 1991.Arch.Toxicol.65, 437.
Martin, S.J., et al. 1990.J. Immunology145, 1859.
Wyllie, A. H. 1980.Nature284, 555.
Kerr, J. F. R., et al. 1972.Br. J. Cancer26, 239.
由于该运输中有害物质的性质，您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系，以获取有关这些费用的更多信息。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Parafilm is a registered trademark of Bemis Company, Inc.
警示用语：
Danger
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B - Eye Irrit. 2
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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