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Merck
CN

PSHT004

Millicell® 96孔板

pore size 0.4 μm, polycarbonate membrane, sterile, Tissue Culture (TC)-treated surface, automation compatible

别名:

细胞培养板

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UNSPSC Code:
41104923
NACRES:
NB.13
eCl@ss:
32039006

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产品名称

Millicell® 96孔板, pore size 0.4 μm, polycarbonate membrane, sterile, Tissue Culture (TC)-treated surface, automation compatible

material

polycarbonate membrane
polystyrene plate

description

  • 1/pk components: 96 well cell culture plate (1), Single well feeder tray (1), 96 well receiver plate (1), Lid (2)
  • 5/pk components: 96 well cell culture plate (5),Single well feeder tray (5), Lid (5)

sterility

sterile
γ-irradiated

shelf life

3 yr

feature

100x106 pores/cm2 (pore density)
 automation compatible

manufacturer/tradename

Millicell®

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable
toxicology assay: suitable

L × W × H

127.76 mm × 85.47 mm × 20.32 mm, with lid

filtration area

0.11 cm2

plate size

96 wells

working volume

0.075 mL

pore size

0.4 μm

Quality Level

400

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

相关类别

General description

Millicell 细胞培养嵌套板是一种专利装置，设计用于支持内皮和上皮细胞系（包括 CaCo-2）的细胞生长和分化。

Millicell 装置有 0.4 µm PCF 和多种 PET 薄膜孔径。培养板给用户提供了最大方便性，有许多特点，例如更加方便移液的上侧辅助孔以及下侧接触孔。为了降低污染细胞单层的风险，薄膜板有支脚，使板子在从饲养托盘拆下时高于工作表面。泪滴形状的容纳孔消除了在组装板子时产生的气泡。

Millicell 培养板经优化用于生长细胞和维持细胞单层的高度完整性。Millicell培养板薄膜上生长的细胞的生长情况比在塑料上好，因为细胞从上侧和下侧均获得营养。更加密切地模拟了体内细胞生长和功能。Millicell 培养板设计用于分析及细胞生长，亦可用作手动或自动细胞接种、饲养和清洗系统。

使用这一完善的系统，您可以在一个以薄膜做底的培养板上生长、饲养和分析细胞。培养板可以与单槽或多孔饲养托盘配用。在进行转运分析时，只需将培养板转换成分析用的多孔转运托盘。

这种流线型设计增强了下列功能：

-接种和饲养系统

-自动液体处理系统和跨距移液

-下侧和上侧均可接触到细胞

-经上皮电阻（TEER）测量系统

Features and Benefits

  • Enhanced Cell Growth: Cells cultured on Millicell plates exhibit better growth compared to traditional plastic, as they receive nutrients from both the apical and basolateral sides, mimicking in vivo conditions.
  • User-Friendly Design: Incorporates apical assist for easy pipetting and basolateral access. Tear drop-shaped receiver wells prevent air bubbles during assembly.
  • Streamlined Workflow: The complete system allows for simultaneous growth, feeding, and analysis within one membrane-bottom plate, simplifying workflows.
  • Automation Compatible: Designed to work seamlessly with seed-and-feed systems, automated liquid handling, and TEER measurement systems, enhancing laboratory efficiency.

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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实验方案

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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