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PCYTMAG-23K

Millipore

MILLIPLEX® 猪细胞因子和趋化因子磁珠面板 - 免疫学多重检测

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology in porcine serum, plasma and cell culture samples.

别名:

luminex porcine cytokine chemokine growth factor multiplex assay, luminex porcine pro inflammatory anti inflammatory cytokine panel, millipore porcine inflammation multiplex kit, porcine interleukin immunoassay panel

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.84

质量水平

200

种属反应性

pig

制造商/商品名称

Milliplex®

assay range

accuracy: 92-103%
standard curve range: 0.005-20 ng/mL
(IL-1α)
standard curve range: 0.012-50 ng/mL
(IL-8)
standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα)
standard curve range: 0.061-250 ng/mL
(IL-4)
standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

一般描述

由于在心脏大小、血液流速、皮肤、肝酶和骨愈合方面与人类相似，猪模型在心血管、毒理学、药理学、衰老和骨骼等许多研究领域取代了许多犬和非人灵长类动物模型。“小型猪”是为了保持与人类相似的心脏大小和功能而饲养的，但年龄不会太大，已用于长期临床前或多代研究，这些研究可能在相对较短的时间内进行。

MILLIPLEX®猪细胞因子/趋化因子检测板可用于同时定量猪组织/细胞裂解物和培养上清液样本以及血清或血浆样本中的以下任何或所有分析物：GM-CSF、IFNγ、IL-1α、IL-1ra、IL-1β、IL-2、IL-4、IL-6、IL-8、IL-10、IL-12、IL-18和TNFα。该试剂盒采用96孔板，包含冻干标准混合物、两个内部检测质控品，最多可测量38份样本，一式两份。

Luminex® xMAP®平台使用磁珠免疫分析格式，以实现理想的速度和灵敏度，同时定量多种分析物，从而显著提高生产力，同时节省有价值的样品量。

面板类型：细胞因子/趋化因子

特异性

抗体与该面板中任何其他分析物之间的交叉反应性都为无法检测或可忽略。

应用

  • 分析物：GM-CSF、IFNγ、IL-1α、IL-1β、IL-1Ra、IL-2、IL-4、IL-6、IL-8（CXCL8）、IL-10、IL-12、IL-18、TNF-α
  • 推荐的样品类型：血清、血浆或细胞/组织培养上清液或裂解液
  • 推荐的样品稀释度：每孔25 μL未稀释的血清或血浆；细胞/组织培养样品可能需要在适当的对照培养基中稀释。
  • 注意：对于脂质含量极高的样品，可能需要额外离心并注意。
  • 分析运行时间：在2-8℃下过夜（16-18小时）。
  • 研究类别：炎症 & 免疫学

特点和优势

通过在此面板中选择可用的分析物来设计多重试剂盒。

储存及稳定性

试剂盒组分建议的存储温度为2-8°C。

其他说明

灵敏度：有关单个生物标志物的敏感性，请参阅试剂盒方案。

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形图

Skull and crossbonesEnvironment
GHS06,GHS09

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

法规信息

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Victoria J Vieira-Potter et al.
Obesity (Silver Spring, Md.), 23(12), 2421-2429 (2015-11-03)
The Ossabaw pig is emerging as an attractive model of human cardiometabolic disease because of its size and susceptibility to atherosclerosis, among other characteristics. The relationship between adipose tissue inflammation and metabolic dysfunction in this model was investigated here. Young
Christina Veit et al.
Frontiers in veterinary science, 7, 617634-617634 (2021-02-16)
Poor health is a risk factor for damaging behaviors, but the mechanisms behind this link are unknown. Injection of pigs with lipopolysaccharide (LPS) can be used to model aspects of poor health. Recent studies have shown that LPS-injected pigs perform
Marina López et al.
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
This work studied the effects of the inclusion of Clostridium butyricum on feed, alone or with carob meal or citrus pulp, on the digestive and metabolic status of weaned piglets. A total of 30 male piglets (weaned at 21 days)
Jessica A Hiemstra et al.
Physiological reports, 2(6) (2014-06-26)
We recently developed a clinically relevant mini-swine model of heart failure with preserved ejection fraction (HFpEF), in which diastolic dysfunction was associated with increased mitochondrial permeability transition (MPT). Early diastolic function is ATP and Ca(2+)-dependent, thus, we hypothesized chronic low
Azita Mellati et al.
Frontiers in immunology, 13, 850271-850271 (2022-06-21)
Ischaemia-reperfusion injury (IRI) is an inevitable process in transplantation and results in inflammation and immune system activation. Alpha-1 antitrypsin (AAT) has anti-inflammatory properties. Normothermic machine perfusion (NMP) can be used to deliver therapies and may help in assessing the effects
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