推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% Sodium azide as preservative
种属反应性
Drosophila, hamster, rat, human, monkey, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.
Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.
This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.
免疫原
a recombinant protein consisting of full-length human CHIP fused to a His•Tag sequence
应用
Immunoblotting (1:200-1:1000)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
警告
Toxicity: Standard Handling (A)
外形
Undiluted serum.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
COS-7 cells
COS-7 cells
其他说明
May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Britney N Lizama et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(31), 6825-6840 (2018-06-24)
The C terminus of HSC70-interacting protein (CHIP, STUB1) is a ubiquitously expressed cytosolic E3-ubiquitin ligase. CHIP-deficient mice exhibit cardiovascular stress and motor dysfunction before premature death. This phenotype is more consistent with animal models in which master regulators of autophagy
Shoshiro Hirayama et al.
Molecular biology of the cell, 19(3), 899-911 (2007-12-21)
McKusick-Kaufman syndrome (MKKS) is a recessively inherited human genetic disease characterized by several developmental anomalies. Mutations in the MKKS gene also cause Bardet-Biedl syndrome (BBS), a genetically heterogeneous disorder with pleiotropic symptoms. However, little is known about how MKKS mutations
Xinjia Wang et al.
Molecular endocrinology (Baltimore, Md.), 19(6), 1474-1482 (2005-03-12)
The ubiquitin/proteasome-dependent protein degradation pathway (UPP) is responsible for the accelerated down-regulation of glucocorticoid receptor (GR) levels in cells subjected to chronic glucocorticoid exposure. Whereas hormone-dependent down-regulation of GR operates in most cells, the receptor is not down-regulated after long-term
Rudolf A Kley et al.
Brain : a journal of neurology, 135(Pt 9), 2642-2660 (2012-09-11)
Mutations in FLNC cause two distinct types of myopathy. Disease associated with mutations in filamin C rod domain leading to expression of a toxic protein presents with progressive proximal muscle weakness and shows focal destructive lesions of polymorphous aggregates containing
Jeannette N Stankowski et al.
Antioxidants & redox signaling, 14(10), 1787-1801 (2010-08-04)
The decision to remove or refold oxidized, denatured, or misfolded proteins by heat shock protein 70 and its binding partners is critical to determine cell fate under pathophysiological conditions. Overexpression of the ubiquitin ligase C-terminus of HSC70 interacting protein (CHIP)
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