产品名称
抗-钙结合蛋白D-28K (Ab-1) 兔pAb, lyophilized, Calbiochem®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
baboon, monkey, rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, )
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CALB1(793)
rhesus monkey ... Calb1(677721)
Analysis Note
阳性对照
大鼠纹状体、海马或皮质
大鼠纹状体、海马或皮质
Application
冷冻切片(整个安装/振动切片机切片,1:1000,荧光)
振动切片机切片(生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000,请参阅应用参考文献)
振动切片机切片(生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000,请参阅应用参考文献)
Disclaimer
毒性:标准处理(A)
General description
兔多克隆抗体以冻干未稀释的血清形式提供。可识别钙结合蛋白D-28K蛋白。
兔多克隆抗钙结合蛋白D-28K(Ab-1)可重新识别大鼠海马中的钙结合蛋白D-28K蛋白。经验证可用于冰冻切片和T细胞共刺激。
可识别大鼠海马组织中的钙结合蛋白D-28K蛋白。
Immunogen
牛
纯化牛小脑钙结合蛋白D-28K蛋白
Other Notes
Conde, F., et al. 1994.J. Comp.Neurol.341, 95.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
在大鼠纹状体,皮层和海马中检查了抗体特异性。根据生产商说明,使用生物素-链霉亲和素/过氧化物酶试剂检测一抗。在单个系统中,应对抗体进行滴定以获得最佳结果。
Physical form
未稀释的血清。
Preparation Note
用100 µl无菌蒸馏H₂O复溶冻干抗体。所得复溶溶液将包含≤0.1%叠氮化钠。注意复溶小瓶的全部内容物;在运输和处理过程中,冻干颗粒的部分可能已脱落,可能不在小瓶底部。用无菌PBS或Tris缓冲液稀释,稀释度不高于1:10。溶解后,等分并冷冻保存(-20°C)。储备溶液在-20°C下可稳定保存至多6个月。避免溶液反复冻融。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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