推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
lyophilized
包含
≤0.1% sodium azide as preservative
种属反应性
baboon, monkey, rat, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
ambient
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CALB1(793)
rhesus monkey ... Calb1(677721)
一般描述
兔多克隆抗体以冻干未稀释的血清形式提供。可识别钙结合蛋白D-28K蛋白。
兔多克隆抗钙结合蛋白D-28K（Ab-1）可重新识别大鼠海马中的钙结合蛋白D-28K蛋白。经验证可用于冰冻切片和T细胞共刺激。
可识别大鼠海马组织中的钙结合蛋白D-28K蛋白。
免疫原
牛
纯化牛小脑钙结合蛋白D-28K蛋白
应用
冷冻切片（整个安装/振动切片机切片，1:1000，荧光）
振动切片机切片（生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000，请参阅应用参考文献）
振动切片机切片（生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000，请参阅应用参考文献）
警告
毒性：标准处理（A）
外形
未稀释的血清。
重悬
用100 µl无菌蒸馏H₂O复溶冻干抗体。所得复溶溶液将包含≤0.1%叠氮化钠。注意复溶小瓶的全部内容物；在运输和处理过程中，冻干颗粒的部分可能已脱落，可能不在小瓶底部。用无菌PBS或Tris缓冲液稀释，稀释度不高于1:10。溶解后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。避免溶液反复冻融。
分析说明
阳性对照
大鼠纹状体、海马或皮质
大鼠纹状体、海马或皮质
其他说明
Conde, F., et al. 1994.J. Comp.Neurol.341, 95.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
在大鼠纹状体，皮层和海马中检查了抗体特异性。根据生产商说明，使用生物素-链霉亲和素/过氧化物酶试剂检测一抗。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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