推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
lyophilized
包含
≤0.1% sodium azide as preservative
种属反应性
baboon, monkey, rat, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
ambient
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CALB1(793)
rhesus monkey ... Calb1(677721)
一般描述
兔多克隆抗体以冻干未稀释的血清形式提供。可识别钙结合蛋白D-28K蛋白。
兔多克隆抗钙结合蛋白D-28K（Ab-1）可重新识别大鼠海马中的钙结合蛋白D-28K蛋白。经验证可用于冰冻切片和T细胞共刺激。
可识别大鼠海马组织中的钙结合蛋白D-28K蛋白。
免疫原
牛
纯化牛小脑钙结合蛋白D-28K蛋白
应用
冷冻切片（整个安装/振动切片机切片，1:1000，荧光）
振动切片机切片（生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000，请参阅应用参考文献）
振动切片机切片（生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000，请参阅应用参考文献）
警告
毒性：标准处理（A）
外形
未稀释的血清。
重悬
用100 µl无菌蒸馏H₂O复溶冻干抗体。所得复溶溶液将包含≤0.1%叠氮化钠。注意复溶小瓶的全部内容物；在运输和处理过程中，冻干颗粒的部分可能已脱落，可能不在小瓶底部。用无菌PBS或Tris缓冲液稀释，稀释度不高于1:10。溶解后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。避免溶液反复冻融。
分析说明
阳性对照
大鼠纹状体、海马或皮质
大鼠纹状体、海马或皮质
其他说明
Conde, F., et al. 1994.J. Comp.Neurol.341, 95.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
在大鼠纹状体，皮层和海马中检查了抗体特异性。根据生产商说明，使用生物素-链霉亲和素/过氧化物酶试剂检测一抗。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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PloS one, 10(9), e0138107-e0138107 (2015-09-30)
Gaucher disease is characterized by lysosomal accumulation of glucosylceramide due to deficient activity of lysosomal glucocerebrosidase (GBA). In cells, glucosylceramide is also degraded outside lysosomes by the enzyme glucosylceramidase 2 (GBA2) of which inherited deficiency is associated with ataxias. The
Ana I Arroyo et al.
EMBO molecular medicine, 6(3), 398-413 (2014-01-23)
Understanding the role of lipids in synapses and the aberrant molecular mechanisms causing the cognitive deficits that characterize most lipidosis is necessary to develop therapies for these diseases. Here we describe sphingomyelin (SM) as a key modulator of the dendritic
B A Patel et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 22(8), 909-918 (2010-05-21)
Inhibitory neurotransmission to the longitudinal muscle is more prominent in the neonatal than in the adult guinea pig ileum. Inhibitory neuromuscular transmission was investigated using in vitro ileal longitudinal muscle myenteric plexus (LMMP) preparations made from neonatal (< or =48
André R A Marques et al.
PloS one, 10(8), e0135889-e0135889 (2015-08-15)
The enzyme glucocerebrosidase (GBA) hydrolyses glucosylceramide (GlcCer) in lysosomes. Markedly reduced GBA activity is associated with severe manifestations of Gaucher disease including neurological involvement. Mutations in the GBA gene have recently also been identified as major genetic risk factor for
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FAT4 mutations lead to several human diseases that disrupt the normal development of the kidney. However, the underlying mechanism remains elusive. In studying the duplex kidney phenotypes observed upon deletion of Fat4 in mice, we have uncovered an interaction between
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