生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
lyophilized
不包含
preservative
种属反应性
rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG
运输
ambient
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
rat ... Gabra1(29705)
一般描述
兔多克隆抗GABA抗体可识别大鼠丘脑和小脑中的GABA。经验证可用于冰冻切片的免疫组织化学。
兔多克隆抗体以冻干未稀释的血清形式提供。可识别GABA。
可识别大鼠丘脑和小脑中的GABA。
免疫原
GABA
大鼠
应用
冰冻切片（1:12,000-1:15,000，ABC检测或1:3000-1:5000，PAP检测；见备注）
警告
毒性：标准处理（A）
外形
未稀释的血清。
重悬
用100 µl无菌蒸馏H₂O复溶冻干抗体。保存时，用无菌PBS或Tris缓冲液稀释，稀释度不高于1:10。复溶小瓶的全部内容物；在运输和处理过程中，冻干颗粒的部分可能已脱落，可能不在小瓶底部。溶解后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。避免溶液反复冻融。
分析说明
阳性对照
大鼠丘脑或小脑
大鼠丘脑或小脑
其他说明
Sudhof, T.C.1995.Nature375, 645.
Hokfelt, T., et al. 1987.Experientia Suppl.56, 154.
Hokfelt, T., et al. 1987.Experientia Suppl.56, 154.
在大鼠丘脑和小脑中检查了抗体特异性。戊二醛是与该抗体一起使用时固定的必要组分。可以根据需要使用更高浓度的戊二醛（1%-2%）。使用竞争性抑制ELISA评价抗体对GABA的特异性。GABA共轭物对照完全消除了标记，而以下共轭物的1000倍过量：丙氨酸，天冬氨酸，β丙氨酸，谷氨酸，甘氨酸，牛磺酸和酪氨酸不抑制与GABA共轭物对照的结合。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our 产品选型工具.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Abram Akopian et al.
The Journal of comparative neurology, 527(1), 159-173 (2016-07-14)
We determined whether the structural and functional integrity of amacrine cells (ACs), the largest cohort of neurons in the mammalian retina, are affected in glaucoma. Intraocular injection of microbeads was made in mouse eyes to elevate intraocular pressure as a
Todd McLaughlin et al.
Investigative ophthalmology & visual science, 64(14), 40-40 (2023-11-28)
Diabetic retinopathy (DR) is a leading cause of blindness in working-age adults characterized by retinal dysfunction and neurovascular degeneration. We previously reported that deletion of X-box binding protein 1 (XBP1) leads to accelerated retinal neurodegeneration in diabetes; however, the mechanisms
Abram Akopian et al.
The Journal of clinical investigation, 127(7), 2647-2661 (2017-06-13)
The progressive death of retinal ganglion cells and resulting visual deficits are hallmarks of glaucoma, but the underlying mechanisms remain unclear. In many neurodegenerative diseases, cell death induced by primary insult is followed by a wave of secondary loss. Gap
Todd McLaughlin et al.
Cells, 12(12) (2023-06-28)
p58IPK is a multifaceted endoplasmic reticulum (ER) chaperone and a regulator of eIF2α kinases involved in a wide range of cellular processes including protein synthesis, ER stress response, and macrophage-mediated inflammation. Systemic deletion of p58IPK leads to age-related loss of
Zheng-Li Fan et al.
PloS one, 14(12), e0227200-e0227200 (2020-01-01)
Schizophrenia (SZ) is a serious and incurable mental disorder characterized by clinical manifestations of positive and negative symptoms and cognitive dysfunction. High-frequency deep brain stimulation (DBS) of the ventral hippocampus (VHP) has been recently applied as a therapeutic approach for
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门