推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
lyophilized
包含
≤0.1% sodium azide as preservative
种属反应性
rat, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG
运输
ambient
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Calca(12310)
一般描述
兔多克隆抗CGRP可识别大鼠杏仁核和脊髓中的α-CGRP。它已被验证可用于免疫荧光，&可与冷冻、石蜡和自由浮动切片一起使用。
兔多克隆抗体以未稀释的血清形式提供。可识别α-CGRP蛋白。
可识别大鼠杏仁核和脊髓中的α-降钙素基因相关肽。
免疫原
大鼠α-降钙素基因相关肽（α-CGRP）
应用
自由浮动切片（参见应用参考文献）
冷冻切片（1:2000-1:4000，ABC检测）
免疫荧光（1:100-1:200，请参阅应用参考文献）
石蜡切片（请参阅应用参考文献；需要预先热处理；参见注释）
警告
毒性：标准处理（A）
重悬
用100 µl无菌蒸馏H₂O复溶冻干抗体。注意复溶小瓶的全部内容物；在运输和处理过程中，冻干颗粒的部分可能已脱落，可能不在小瓶底部。溶解后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。用无菌PBS或Tris缓冲液稀释，稀释度不高于1:10。避免溶液反复冻融。
分析说明
阳性对照
大鼠杏仁核或脊髓
大鼠杏仁核或脊髓
其他说明
在大鼠杏仁核和脊髓中检查了抗体特异性。用10-5M大鼠α-CGRP预处理抗体可完全消除染色，用大鼠β-CGRP预吸收可部分消除染色。降钙素的预吸收不会导致免疫染色的损失。对于石蜡切片染色，将切片在40 mM枸橼酸钠缓冲液（pH 8.5）中加热至80°C。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Stuart M Brierley et al.
Gastroenterology, 134(7), 2059-2069 (2008-03-18)
Although there are many candidates as molecular mechanotransducers, so far there has been no evidence for molecular specialization of visceral afferents. Here, we show that colonic afferents express a specific molecular transducer that underlies their specialized mechanosensory function: the transient
D J Van Daele et al.
Neuroscience, 162(2), 501-524 (2009-05-12)
The present study investigated the central connections of motor neurons innervating the thyroarytenoid laryngeal muscle that is active in swallowing, respiration and vocalization. In both intact and sympathectomized rats, the pseudorabies virus (PRV) was inoculated into the muscle. After initial
Francis M Hughes et al.
Diabetes, 68(2), 430-440 (2018-11-15)
The NLRP3 inflammasome senses diabetic metabolites and initiates inflammation implicated in diabetic complications and neurodegeneration. No studies have investigated NLRP3 in diabetic bladder dysfunction (DBD), despite a high clinical prevalence. In vitro, we found that numerous diabetic metabolites activate NLRP3
Paula Dietrich et al.
Human molecular genetics, 21(23), 5078-5090 (2012-08-28)
Hereditary sensory and autonomic neuropathies (HSANs) encompass a group of genetically inherited disorders characterized by sensory and autonomic dysfunctions. Familial dysautonomia (FD), also known as HSAN type III, is an autosomal recessive disorder that affects 1/3600 live births in the
Min-Hee Oh et al.
Journal of immunology (Baltimore, Md. : 1950), 191(11), 5371-5382 (2013-10-22)
Chronic debilitating pruritus is a cardinal feature of atopic dermatitis (AD). Little is known about the underlying mechanisms. Antihistamines lack efficacy in treating itch in AD, suggesting the existence of histamine-independent itch pathways in AD. Transient receptor potential ankyrin 1
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