产品名称
抗-Rad51 (Ab-1) 兔pAb, liquid, Calbiochem®
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (1:2500)
Immunoprecipitation (40 µL/500µg total protein from lysate)
Immunocytochemistry (1:500))
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RAD51(5888)
Analysis Note
阳性对照
A431细胞，HeLa细胞
A431细胞，HeLa细胞
Application
免疫印迹法（1:2500）
免疫沉淀（40 L/500g裂解物总蛋白）
免疫细胞化学（1:500）
Disclaimer
毒性：标准处理（A）
General description
亲和纯化的兔多克隆抗体。可识别~43 kDa Rad51蛋白。
细菌RecA和酵母RAD51蛋白参与同源重组和DNA修复。人RAD51蛋白是这两种蛋白的同源物。尽管这些蛋白质的模块化结构是保守的，并且与细菌蛋白质的同源性非常强，但RAD51存在功能差异，包括RecA所见的ATP水解速率为1/10。尽管RAD51是一种核蛋白，在细胞质和细胞核中基本上不存在，但该蛋白缺乏核定位信号，需要复合物中的其他蛋白才能转移到细胞核中。用DNA合成诱导剂处理细胞将导致RAD51蛋白水平增加，然后经历分布变化，成为自然界的焦点。这种局部分布可能被转录抑制剂阻断。RAD51可以直接与染色体相互作用，并且在减数分裂过程中，RAD51的分布发生动态变化。已显示RAD51与多种不同的蛋白质相互作用，包括野生型形式的p53、BRCA2和RAD52，它们特异性调节其活性。
识别卵巢组织中约43 kDa的Rad51蛋白。
该抗Rad51（Ab-1）兔pAb经验证可用于免疫印迹、免疫沉淀和免疫细胞化学以检测Rad51（Ab-1）。
Immunogen
人
重组人Rad51
Other Notes
Brendel, V., et al. 1997. J. Mol.Evol.44, 528.
Boulikas, T., et al. 1997.Anticancer Res.17, 843.
Gupta, R.C., et al. 1997.Proc.Natl.Acad.Sci. USA94, 463.
Mizuta, R., et al. 1997.Proc.Natl.Acad.Sci. USA 94, 6927.
Sharan, S.K., et al. 1997.Nature386, 304.
Li, M.J., et al. 1996.Proc.Natl.Acad.Sci. USA93, 10222.
Shen, Z., et al. 1996.J. Biol. Chem.271, 148.
Sturzbecher, H.W., et al. 1996.EMBO J. 15, 1992.
Tashiro, S., et al. 1996.Oncogene12, 2165.
Ashley, T., et al. 1995.Chromosoma104, 19.
Haaf, T., et al. 1995.Proc.Natl.Acad.Sci. USA92, 2298.
Boulikas, T., et al. 1997.Anticancer Res.17, 843.
Gupta, R.C., et al. 1997.Proc.Natl.Acad.Sci. USA94, 463.
Mizuta, R., et al. 1997.Proc.Natl.Acad.Sci. USA 94, 6927.
Sharan, S.K., et al. 1997.Nature386, 304.
Li, M.J., et al. 1996.Proc.Natl.Acad.Sci. USA93, 10222.
Shen, Z., et al. 1996.J. Biol. Chem.271, 148.
Sturzbecher, H.W., et al. 1996.EMBO J. 15, 1992.
Tashiro, S., et al. 1996.Oncogene12, 2165.
Ashley, T., et al. 1995.Chromosoma104, 19.
Haaf, T., et al. 1995.Proc.Natl.Acad.Sci. USA92, 2298.
由于高度的序列同一性，预期该抗体也可与牛、马和犬RAD51发生交叉反应。应对单个系统的抗体进行滴定。
Physical form
溶于150 mM NaCl，100 mM Tris-甘氨酸，pH 7.4
Preparation Note
初次融化后，等分并冷冻（-20°C）。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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