推荐产品
一般描述
原癌基因fos与细胞的生长，分化和发育有关。Fos是由大量刺激物诱导的，包括有丝分裂原，分化特异性药物，药理药物等。62,000道尔顿fos蛋白的诱导是快速但短暂的。fos蛋白与39,000道尔顿蛋白相关，该蛋白已被鉴定为jun原癌基因（c-jun）的蛋白产物。Fos/Jun蛋白复合物与DNA中称为AP-1结合位点的序列元件特异性结合。
可识别HepG2、NIH3T3和MCF-7细胞中的~50-62 kDa c-Fos蛋白。
蛋白A纯化的兔多克隆抗体。可识别v-fos和~50-62 kDa c-fos蛋白。
该抗c-Fos（Ab-2）（4-17）兔pAb经验证可用于冰冻切片、免疫印迹、IF、IP、石蜡切片，以检测c-Fos（Ab-2）（4-17）。
免疫原
人
对应于人c-Fos氨基酸4-17的合成肽（SGFNADYEASSSRC）（目录号PP10）
应用
冰冻切片（5-10 µg/ml）
免疫印迹（1-5 µg/ml）
免疫荧光（1-5 µg/ml）
免疫沉淀（1-2 µg/反应）
石蜡切片（5-10 µg/ml，需要胃蛋白酶或加热预处理）
自由浮动切片（不建议使用）
免疫印迹（1-5 µg/ml）
免疫荧光（1-5 µg/ml）
免疫沉淀（1-2 µg/反应）
石蜡切片（5-10 µg/ml，需要胃蛋白酶或加热预处理）
自由浮动切片（不建议使用）
包装
请参考特定浓度批号的标签。
警告
毒性：高毒性（H）
外形
溶于0.05 M磷酸钠缓冲液，含0.2％明胶。
分析说明
阳性对照
HepG2、NIH 3T3细胞、MCF-7细胞、结肠或乳腺腺癌组织
HepG2、NIH 3T3细胞、MCF-7细胞、结肠或乳腺腺癌组织
其他说明
DeTogni, P., et al. 1988.Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988.Science240, 1010.
Sassone-Corsi, P., et al. 1988.Cell54, 553.
Verma, I.M. and Graham, W.R.1987.Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987.Cell51, 513.
Muller, R. 1986.Biochim.Biophys.Acta823, 207.
Verma, I. 1986.Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984.Nature (London), 311, 433.
Rouscher III, F.J., et al. 1988.Science240, 1010.
Sassone-Corsi, P., et al. 1988.Cell54, 553.
Verma, I.M. and Graham, W.R.1987.Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987.Cell51, 513.
Muller, R. 1986.Biochim.Biophys.Acta823, 207.
Verma, I. 1986.Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984.Nature (London), 311, 433.
对于凝胶阻滞分析，使用目录编号PC05L重悬于100 µl缓冲液中。免疫原，c-fos（肽-2），目录编号PP10也可用于竞争研究（请参见下面的Murphy等人）。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
10 - Combustible liquids
WGK
nwg
法规信息
新产品
A R Soderman et al.
Neuroscience, 154(4), 1506-1516 (2008-06-14)
Opioid receptor agonists and antagonists have profound effects on cocaine-induced hyperactivity and conditioned reward. Recently, the role specifically of the mu opioid receptor has been demonstrated based on the finding that i.c.v. administration of the selective mu opioid receptor antagonist
N Laflamme et al.
Journal of molecular neuroscience : MN, 8(3), 165-179 (1997-06-01)
The purpose of this study was to investigate the effect of the immune activator lipopolysaccharide (LPS) on the expression of corticotropin-releasing factor (CRF) and glucocorticoid receptor (GR) mRNA in the paraventricular nucleus (PVN) of transgenic mice with impaired GR function
Natasha M Sosanya et al.
BMC neuroscience, 20(1), 17-17 (2019-04-25)
Reports show that stressful events before injury exacerbates post-injury pain. The mechanism underlying stress-induced heightened thermal pain is unclear. Here, we examined the effects of chronic intermittent stress (CIS) on nociceptive behaviors and brain-derived nerve growth factor (BDNF) system in
Clifford S Deutschman et al.
Critical care medicine, 41(11), e368-e375 (2013-10-10)
Sepsis is a common, lethal poorly understood disorder affecting nearly a million Americans annually. The syndrome is characterized by altered cardiodynamics, respiration, metabolism, pituitary function, arousal, and impaired interaction among organ systems. The immunologic and endocrine systems, which are in
Andrew D Chang et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 43(5), 1088-1098 (2017-10-21)
Growing recognition of persistent cognitive defects associated with electroconvulsive therapy (ECT), a highly effective and commonly used antidepressant treatment, has spurred interest in identifying its mechanism of action to guide development of safer treatment options. However, as repeated seizure activity
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门