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Merck
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安全信息

PAT001

Sigma-Aldrich

Protein A-Turbo - Proximity Biotinylation Enzyme

别名:

ProteinA-Turbo ID

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.46

表单

liquid

质量水平

纯化方式

affinity chromatography

包装

pkg of 100 μg

技术

affinity binding assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
mass spectrometry (MS): suitable
proximity ligation assay: suitable

储存温度

-65 to -96°C

一般描述

Protein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.

特异性

Protein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.

应用

  • Proximity protein labeling
  • Cell Biology
  • Histology
  • Fixed cell microscopy
  • Tissue staining
  • Immunofluorescence
  • Liquid Chromatography
  • Mass Spectrometry
  • Immunoprecipitation


外形

Purified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.

重悬

0.7 mg/mL

储存及稳定性

Store at -65°C to -96°C.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Health hazardExclamation mark

警示用语:

Danger

危险声明

危险分类

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Irene Santos-Barriopedro et al.
Nature communications, 12(1), 5015-5015 (2021-08-20)
Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins or post-translational modifications

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