推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
TA-1, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
请勿与下列物质发生反应
mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... ERBB2(2064)
一般描述
Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.
Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.
应用
Flow Cytometry (5 µg/ml, see application references)
Frozen Sections (5 µg/ml)
Immunoblotting (see application references)
Immunofluorescence (5 µg/ml, see application references)
Immunoprecipitation (1 µg/sample, see application references)
Neutralization Studies (not recommended, see comments and application references)
Frozen Sections (5 µg/ml)
Immunoblotting (see application references)
Immunofluorescence (5 µg/ml, see application references)
Immunoprecipitation (1 µg/sample, see application references)
Neutralization Studies (not recommended, see comments and application references)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 50 mM PBS, 0.2% gelatin, pH 7.5.
分析说明
Positive Control
SK-BR-3 cells
SK-BR-3 cells
其他说明
DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.
Bargmann, C.I., et al. 1968. Nature319, 226.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.
Bargmann, C.I., et al. 1968. Nature319, 226.
Recognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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