推荐产品
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.
Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.
This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).
免疫原
Human
a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
应用
Immunoblotting (2.5-5 µg/ml)
Immunocytochemistry (2.5-5 µg/ml)
Paraffin Sections (not recommended)
Immunocytochemistry (2.5-5 µg/ml)
Paraffin Sections (not recommended)
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from a volatile buffer, 200 µg BSA.
重悬
Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.
分析说明
Positive Control
HeLa cells
HeLa cells
其他说明
Jensen, D.E., et al. 1991. Oncogene6, 1219.
Reiss, M., et al. 1990. Cancer Res.50, 6641.
RouscherIII, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res.49, 29.
Verma, I.M., et al. 1987. Cell51, 513.
Muller, R. 1986. Viochem. Biophys. Acta.823, 207.
Verma, P. 1986. Trends Genet.2, 93.
Greenberg, M., et al. 1984. Nature (London)311, 433.
Reiss, M., et al. 1990. Cancer Res.50, 6641.
RouscherIII, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res.49, 29.
Verma, I.M., et al. 1987. Cell51, 513.
Muller, R. 1986. Viochem. Biophys. Acta.823, 207.
Verma, P. 1986. Trends Genet.2, 93.
Greenberg, M., et al. 1984. Nature (London)311, 433.
Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
11 - Combustible Solids
WGK
WGK 1
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门