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Merck
CN
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文件

OP17L

Sigma-Aldrich

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

lyophilized, clone 2G9C3, Calbiochem®

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UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

100

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

2G9C3, monoclonal

形式

lyophilized

不包含

preservative

种属反应性

rat, human, mouse

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

同位素/亚型

IgG2a

运输

ambient

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... FOS(2353)

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.
Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.
This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

免疫原

Human
a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos

应用

Immunoblotting (2.5-5 µg/ml)

Immunocytochemistry (2.5-5 µg/ml)

Paraffin Sections (not recommended)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from a volatile buffer, 200 µg BSA.

重悬

Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.

分析说明

Positive Control
HeLa cells

其他说明

Jensen, D.E., et al. 1991. Oncogene6, 1219.
Reiss, M., et al. 1990. Cancer Res.50, 6641.
RouscherIII, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res.49, 29.
Verma, I.M., et al. 1987. Cell51, 513.
Muller, R. 1986. Viochem. Biophys. Acta.823, 207.
Verma, P. 1986. Trends Genet.2, 93.
Greenberg, M., et al. 1984. Nature (London)311, 433.
Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

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