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安全信息

OP133

Sigma-Aldrich

Anti-IKKα Mouse mAb (14A231)

liquid, clone 14A231, Calbiochem®

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UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

14A231, monoclonal

形式

liquid

包含

≤0.1% sodium azide as preservative

种属反应性

monkey, human, mouse

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG1

运输

wet ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... CHUK(1147)

一般描述

Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
Recognizes the ~85 kDa IKKα protein in Daudi cells.
This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα.

免疫原

Human
a recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag sequence

应用


Immunoblotting (1-2 g/ml, chemiluminescence)

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外形

In PBS, 0.2% gelatin, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
Daudi cells

其他说明

Antibody should be titrated for optimal results in individual systems.
Zandi, E., et al. 1998 Science281, 1360.
Didonato, J.A., et al. 1997 Nature388, 548.
Lee, F.S., et al. 1997 Cell88, 213.
Mercurio, F., et al. 1997 Science278, 860.
Regnier, C.H., et al. M. 1997 Cell90, 373.
Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA94, 11758.
Verma, I.M., et al. 1995 Genes. Dev.9, 2723.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

nwg

法规信息

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Faiyaz Ahmad et al.
Scientific reports, 6, 28056-28056 (2016-06-21)
Activation of inflammation in white adipose tissue (WAT), includes infiltration/expansion of WAT macrophages, contributes pathogenesis of obesity, insulin resistance, and metabolic syndrome. The inflammasome comprises an intracellular sensor (NLR), caspase-1 and the adaptor ASC. Inflammasome activation leads to maturation of
Jonathan M Starkey et al.
Diabetes, 55(5), 1252-1259 (2006-04-29)
Evidence of diabetes-induced nuclear factor-kappaB (NF-kappaB) activation has been provided with DNA binding assays or nuclear localization with immunohistochemistry, but few studies have explored mechanisms involved. We examined effects of diabetes on proteins comprising NF-kappaB canonical and noncanonical activation pathways
Michael L Kamradt et al.
Cell death & disease, 12(3), 271-271 (2021-03-17)
Cancers, including glioblastoma multiforme (GBM), undergo coordinated reprogramming of metabolic pathways that control glycolysis and oxidative phosphorylation (OXPHOS) to promote tumor growth in diverse tumor microenvironments. Adaptation to limited nutrient availability in the microenvironment is associated with remodeling of mitochondrial
Cristina Aguilera et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(47), 16537-16542 (2004-11-13)
The NF-kappaB pathway plays a pivotal role in proliferation, differentiation, apoptosis, and immune responses in mammals. The NF-kappaB inhibitor, IkappaB, has classically been characterized for its ability to sequester NF-kappaB transcription factors in the cytoplasm. Nevertheless, a nuclear fraction of
Lindsay Bennett et al.
International journal of cancer, 140(7), 1633-1644 (2016-12-23)
The aim of our study was to examine the relationship between tumour IKKα expression and breast cancer recurrence and survival. Immunohistochemistry was employed in a discovery and a validation tissue microarray to assess the association of tumour IKKα expression and

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