推荐产品
生物来源
rat
质量水平
抗体产品类型
primary antibodies
克隆
Y13-259, monoclonal
表单
suspension (Liquid)
包含
≤0.1% sodium azide as preservative
种属反应性
rat, mouse, human
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
可识别SW480和Y1细胞以及正常皮肤组织中的~21 kDa ras蛋白。对于中和研究，推荐使用目录编号OP01L。对于免疫沉淀，推荐使用目录编号OP01A。
经验证，该抗v-H-Ras（Ab-1）大鼠mAb（Y13-259）琼脂糖偶联物可用于免疫沉淀，检测v-H-Ras（Ab-1）的其他应用。
通过用指定的免疫原免疫Sprague Dawley大鼠并将脾细胞与Y3Ag1.2.3大鼠骨髓瘤细胞融合而产生的大鼠单克隆抗体。与琼脂糖结合。可识别〜21 kDa ras蛋白（请参阅备注）。
免疫原
重组p21 Ras
应用
免疫沉淀（每个样品15 µl珠或1 µg抗体，请参阅备注）
其他应用（请参阅备注）
其他应用（请参阅备注）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外形
溶于PBS。
分析说明
阳性对照
SW 480或Y1细胞或正常皮肤组织
SW 480或Y1细胞或正常皮肤组织
其他说明
Shimizu, K., et al. 1983.Proc.Natl.Acad.Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
推荐使用琼脂糖偶联物进行免疫沉淀。对于冰冻切片、石蜡切片、免疫印迹、中和和免疫荧光，使用目录编号OP01或OP01L。通过免疫沉淀与vH-ras和v-K-ras的磷酸化和非磷酸化形式具有广泛的反应性（请参阅应用参考），并与H-，K-和N-ras人类基因的p21翻译产物发生反应（请参阅应用参考）。该抗体不与rap发生交叉反应。通过与残基Glu-63，Ser-65，Ala-66，Met-67，Gln-70和Arg-73结合来中和脊椎动物H-、K-和N-ras p21的生物学和生化活性（请参阅应用参考）。在用另一种抗体对p21ras进行蛋白质印迹分析之前，建议使用15 µl琼脂糖偶联物作为免疫浓缩剂。为了与未偶联抗体进行免疫沉淀，使用蛋白A与兔抗大鼠IgG或蛋白G PLUS与山羊抗大鼠IgG。单独的蛋白A不会免疫沉淀大鼠IgG。可以检测到对应于具有和不具有法呢基基团的ras蛋白的双峰。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
K Fukuda et al.
Hepatology (Baltimore, Md.), 28(3), 796-804 (1998-09-10)
Transforming growth factor betas (TGF-betas) are the potent growth inhibitors for various cell types. Certain transformed cells, however, show poor response to TGF-beta-induced growth inhibition, which contributes to their uncontrolled proliferation. Recently, we have reported that TGF-beta1 induces degradation of
Lauren M Adams et al.
The Journal of biological chemistry, 299(1), 102768-102768 (2022-12-06)
The KRAS gene is one of the most frequently mutated oncogenes in human cancer and gives rise to two isoforms, KRAS4A and KRAS4B. KRAS post-translational modifications (PTMs) have the potential to influence downstream signaling. However, the relationship between KRAS PTMs
S Zheng et al.
Oncogene, 26(48), 6896-6904 (2007-05-09)
Lung cancer is a devastating disease with poor prognosis. The design of better therapies for lung cancer patients would be greatly aided by good mouse models that closely resemble the human disease. Unfortunately, current models for lung adenocarcinoma are inadequate
Hui Jing et al.
eLife, 6 (2017-12-15)
Ras proteins play vital roles in numerous biological processes and Ras mutations are found in many human tumors. Understanding how Ras proteins are regulated is important for elucidating cell signaling pathways and identifying new targets for treating human diseases. Here
Jia-Hui Wang et al.
The American journal of pathology, 180(1), 82-90 (2011-11-15)
Tubulointerstitial fibrosis is the hallmark of chronic kidney disease and is characterized by an increase in the number and activity of interstitial fibroblasts and by excessive matrix deposition. Ras is an intracellular signaling molecule involved in cell proliferation and differentiation.
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