推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
C3-33, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
fish, canine, rat, avian, amphibian, rabbit
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
rat ... Ryr2(689560)
一般描述
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X 63 AG8.653 mouse myeloma cells (see application references). Supplied as protein G purified antibody. Recognizes the ~565 kDa ryanodine receptor.
Recognizes the ~565 kDa ryanodine receptor in rat brain hippocampus. A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates.
This Anti-Ryanodine Receptor Mouse mAb (C3-33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation for the detection of Ryanodine Receptor.
免疫原
Canine
canine cardiac ryanodine receptor (RYR)
应用
Frozen Sections (1 g/ml, see application references)
Immunoblotting (1 g/ml)
Immunoprecipitation (see comments)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In PBS.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Rat brain hippocampus
Rat brain hippocampus
其他说明
A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.
Sitsapesan, R., et al. 1995. Trends Pharmacol. Sci.16, 386.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem.268, 13765.
Meszaros, L.G., et al. 1993. Nature364, 76.
Lai, F.A., et al. 1992. Biochem. J.288, 553.
Lai, F.A., et al. 1989. Nature331, 315.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem.268, 13765.
Meszaros, L.G., et al. 1993. Nature364, 76.
Lai, F.A., et al. 1992. Biochem. J.288, 553.
Lai, F.A., et al. 1989. Nature331, 315.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Carmen Tarifa et al.
JACC. Basic to translational science, 8(1), 1-15 (2023-02-14)
Analysis of the spatio-temporal distribution of calcium sparks showed a preferential increase in sparks near the sarcolemma in atrial myocytes from patients with atrial fibrillation (AF), linked to higher ryanodine receptor (RyR2) phosphorylation at s2808 and lower calsequestrin-2 levels. Mathematical
Ioannis Smyrnias et al.
Cell calcium, 47(3), 210-223 (2010-01-29)
Narrow, tubular, inward projections of the sarcolemma ('T-tubules') are an established feature of adult mammalian ventricular myocytes that enables them to generate the whole-cell Ca2+ transients and produce coordinated contraction. Loss of T-tubules can occur during ageing and under pathological
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门