storage temp.
2-8°C
Quality Level
biological source
mouse
antibody product type
primary antibodies
clone
SMI-32, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
General description
该产品已停产。
我们提供抗神经丝 H 非磷酸化小鼠单克隆抗体（SMI-32）（货号 559844）作为可能的替代品。请仔细阅读替代产品文档，如果需要其他信息，请联系技术服务部门。
识别大鼠中枢神经系统（CNS）细胞骨架制剂中非磷酸化的 〜180 kDa-200 kDa 神经丝 H 蛋白。
小鼠单克隆抗体以未稀释的腹水形式提供。识别 〜180-200 kDa 非磷酸化神经丝 H 蛋白。
Immunogen
大鼠
来自 Fischer 344 大鼠脑下丘脑匀浆
Application
ELISA (1:1000)
冷冻切片（1:1000，参见注释）
免疫印迹（1:1000，参见注释）
免疫细胞化学（1:1000，参见注释）
石蜡切片（1:1000，需要预先热处理，参见注释）
Physical form
未稀释的腹水。
Preparation Note
初次解冻后，等分并冻存（-20°C）。
Analysis Note
阳性对照
大鼠脑或中枢神经系统细胞骨架制剂
大鼠脑或中枢神经系统细胞骨架制剂
Other Notes
Trapp, B.D., et al. 1998.N. Engl. J. Med.338, 278.
King, C.E., et al. 1997.Neuroreport.8, 1663.
Campbell, M.J., et al. 1991.Brain Res.539, 133.
Campbell, M.J., et al. 1989.J. Comp.Neurol.282, 191.
Sternberger, L.A., et al. 1983.Proc.Natl.Acad.Sci. USA80, 6126.
King, C.E., et al. 1997.Neuroreport.8, 1663.
Campbell, M.J., et al. 1991.Brain Res.539, 133.
Campbell, M.J., et al. 1989.J. Comp.Neurol.282, 191.
Sternberger, L.A., et al. 1983.Proc.Natl.Acad.Sci. USA80, 6126.
仅识别未磷酸化的神经丝 H。通过免疫细胞化学，该抗体可对中枢神经系统和周围神经系统的神经元细胞体、树突和一些浓密的轴突进行染色，而不能对细的轴突或其他细胞和组织进行染色。组织和培养的细胞可以固定在各种含多聚甲醛或甲醛的固定液，例如 Bouin′s 固定液。戊二醛/低聚甲醛固定的样品中抗体反应性差。为了对福尔马林固定的石蜡切片进行染色，建议将脱石蜡的组织在 dH2O 中高压灭菌 10 分钟，或置于 Tris 缓冲盐水（pH 9.0）中在微波炉中煮沸 15 分钟。胰蛋白酶预处理消除了抗体与表位的结合。在冷甲醇或甲醇/过氧化氢中的后固定可促进抗体进入冷冻切片或在4％ 多聚甲醛中固定的厚切片以及培养细胞中的神经元。通过免疫印迹，该抗体检测到两条带，〜180 和 〜200 kDa，在二维凝胶上融合为一条 NFH 线。应在每个系统中对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
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存储类别
10-13 - German Storage Class 10 to 13
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