NE1004
Anti-Apolipoprotein E Mouse mAb (E6D7)
liquid, clone E6D7, Calbiochem®
别名:
Anti-ApoE
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
E6D7, monoclonal
Species reactivity:
human
Application:
Citations:
6
产品名称
Anti-Apolipoprotein E Mouse mAb (E6D7), liquid, clone E6D7, Calbiochem®
biological source
mouse
antibody form
culture supernatant
antibody product type
primary antibodies
clone
E6D7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
should not react with
mouse, rabbit
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1:100-1:1000)
Immunoprecipitation
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... APOE(348)
Analysis Note
Positive Control
Brain tissue, serum
Brain tissue, serum
Application
ELISA (see comments)
Immunoblotting (1:100-1:1000)
Immunoprecipitation (see comments)
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.
Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.
Immunogen
Human
a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
Other Notes
Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
Culture supernatant diluted in PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Abraham Kuot et al.
Clinical & experimental ophthalmology, 47(8), 1028-1042 (2019-06-18)
Fuchs endothelial corneal dystrophy (FECD) is a progressive and potentially a sight threatening disease, and a common indication for corneal grafting in the elderly. Aberrant thickening of Descemet's membrane, formation of microscopic excrescences (guttae) and gradual loss of corneal endothelial
Veena Theendakara et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(3), 685-700 (2016-01-23)
A major unanswered question in biology and medicine is the mechanism by which the product of the apolipoprotein E ε4 allele, the lipid-binding protein apolipoprotein E4 (ApoE4), plays a pivotal role in processes as disparate as Alzheimer's disease (AD; in
Dale J Christensen et al.
Journal of immunology (Baltimore, Md. : 1950), 186(4), 2535-2542 (2011-02-04)
The molecular mechanism by which apolipoprotein E (apoE) suppresses inflammatory cytokine and NO production is unknown. Using an affinity purification approach, we found that peptide mimetics of apoE, derived from its receptor binding domain residues 130-150, bound to the SET
Jing Zhang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(6), 7748-7757 (2019-03-22)
Aging and apolipoprotein E4 (ApoE4) can increase the risk of cognitive impairment and neurodegenerative disorders, including Alzheimer's disease (AD), and patients with type 2 diabetes mellitus are highly susceptible to cognitive dysfunction. Recent research has indicated that metformin, a prescribed
Xia Hu et al.
Frontiers in neuroanatomy, 11, 45-45 (2017-06-24)
Genetic variations in the vacuolar protein sorting 10 protein (Vps10p) family have been linked to Alzheimer's disease (AD). Here we demonstrate deposition of fragments from the Vps10p member sortilin at senile plaques (SPs) in aged and AD human cerebrum. Sortilin
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