Nerve Growth Factor 7.0s

Specific Activity: The neurotrophic activity is in the range of 0.5-50 ng/mL using rat PC12 cells cultured for 7-14 days

The recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100 ng/mL medium (Varon et al., 1967).

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In the mouse submaxillary gland the neurotrophically active NGF, beta-NGF (2.5S), is part of a larger protein complex, 7S-NGF, which has the stoichiometry alpha2betagamma2 (+ 1 or 2 zinc ions) where the alpha and gamma subunits are members of the glandular kallikrein family of serine proteases, each of about 26 000 kDa molecular weight. The alpha and gamma subunits actually inhibit beta-NGF action and must be dissociated for biological activity to be observed.



This 7S complex has not been shown to occur in other mouse tissues, or in other species, probably because the alpha and gamma subunits, which are members of a larger glandular kallikrein family, apparently have a very restricted expression. The alpha and gamma subunits, members of the serine proteinase family, have quite similar amino acid sequences, although only the gamma subunit is catalytically active. The alpha subunit has undergone several mutations that preclude this function, including loss of the cleavage site needed for processing from the zymogen to the active form.

MOLECULAR WT.:

130-140 kD. 7S NGF is composed of 3 subunits; 1 beta subunit (2.5S), 2 alpha subunits and 2 gamma subunits.

Product Source: Mouse submaxillary gland (Darling & Shooter, 1984).

Lyophilized. NC010 should be reconstituted with sterile DMEM at a concentration of

10 μg/mL and stored at -70°C. Medium with mNGF cannot be reused due to mNGF degradation.

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

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