生物来源
mouse
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
162 33 D3, monoclonal
形式
liquid
不包含
preservative
种属反应性（根据同源性预测）
all
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
免疫亲和纯化的小鼠单克隆抗体。识别5-甲基胞嘧啶。
经验证，该抗5-甲基胞嘧啶小鼠mAb（162 33 D3）可用于ELISA、FC、冷冻切片、IF、石蜡切片、放射免疫分析、DNA-蛋白质印迹法检测5-甲基胞嘧啶。
识别NIH3T3细胞中甲基化DNA或RNA中的5-甲基胞嘧啶。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
免疫原
5-甲基胞嘧啶结合卵清蛋白
应用
ELISA（0.1 g/mL）
流式细胞术（1 g/mL）
冷冻切片（5-10 g/mL）
免疫印迹法（5 g/mL）
免疫荧光（1 g/mL）
石蜡切片（5-10 g/mL）
放射免疫分析（0.1 g/mL）
DNA-蛋白质印迹法（5 g/mL）
包装
批次特定浓度请参见小瓶标签。
警告
毒性：标准处理（A）
外形
在PBS（pH 7.4）中。
重悬
初次解冻后，等分并冷冻（-20°C）。
分析说明
阳性对照
NIH3T3细胞
NIH3T3细胞
其他说明
Ehrlich, M. 2002.Oncogene21, 5400.(Review)
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
克隆162 33 D3可用于定量和定性检测各种样品和应用中的甲基化DNA或RNA。它已在40多个出版物中使用。应滴定抗体，以在单个系统中获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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