推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
PC10, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
rat, yeast, mouse, human, zebrafish
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG2a
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... PCNA(5111)
zebrafish ... Pcna(30678)
一般描述
识别HeLa细胞以及扁桃体或正常结肠组织中的~37 kDa PCNA蛋白。
该抗PCNA（Ab-1）小鼠mAb（PC10）经验证可用于免疫印迹、免疫荧光、免疫沉淀石蜡切片以检测PCNA（Ab-1）。
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与Sp2/0-Ag14小鼠骨髓瘤细胞融合而产生的纯化的小鼠单克隆抗体。识别~37 kDa PCNA蛋白。
免疫原
表位：氨基酸112-121内
重组PCNA
应用
免疫印迹（2.5 µg/mL）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
包装
批次特定浓度请参见小瓶标签。
警告
毒性：标准处理（A）
分析说明
阳性对照
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
其他说明
Waseem, N.H. and Lane, D.P.1990.J. Cell.Sci.96, 121.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
染色显示出强烈的核模式。不建议用于免疫沉淀，因为在某些样品中会沉淀出约37 kDa的蛋白质，而不是PCNA。通常，石蜡切片染色不需要预处理，但是在某些样品中，可以通过在37°C下用4 N HCl预处理10分钟，然后在水中洗涤3次来增强染色。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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