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Merck
CN
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MAIPS4510

Millipore

Multiscreen® 96孔板，疏水性PVDF膜

pore size 0.45 μm, sterile

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UNSPSC代码:
41104923
eCl@ss:
32039006
NACRES:
NB.22

物料

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

质量水平

400

描述

Sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

无菌性

sterile

产品线

MultiScreen®

特点

hydrophobic
lid

制造商/商品名称

MultiScreen®

参数

50-250 μL sample volume (per well)

技术

ELISPOT: suitable

过滤面积

0.28 cm2

板尺寸

96 wells

孔最大体积

300 μL

工作体积

50-250 μL

基质

Immobilon®-P

孔径

0.45 μm pore size

输入

sample type cardiac stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type epithelial cells
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)

吸附类型

high binding surface

运输

ambient

相关类别

一般描述

无论您’的应用是什么，密理博都为满足您的具体应用需求开发出适用的过滤板。MultiScreen过滤板长期以来一直都是促进药物研发和生命科学研究的可靠工具。

HTS 过滤板（以 MS 开头的货号）
- MultiScreenHTS过滤板专为自动工作站的大呑吐量应用而开发。坚固的侧壁更易于使用，充裕的表面提供了贴条形码标签的地方。每个板孔都单独密封，防止培养基互扰，而且下排水是可以取下的，从那里可以接触到滤膜。过滤板有96孔和384孔平台，具有广泛的膜和塑料选择。

- Millipore’s的新型MultiScreen HTS+大流量过滤板设计减轻了非特异性结合，减小了背景噪音和信号强度的差异，尤其是对生化筛选测定。

传统过滤板（以 MA 开头的板货号）
- 传统的多孔设计对要求将滤膜浸入测定皿的许多应用特别有价值。易于拆卸的下排水管和独特的板设计是Elispot、PAMPA、细胞筛选（迁移、侵袭或趋化性）或topcount检测用户等分析的理想选择。

配件 – MultiScreenHTS真空多联装置
- MultiScreenHTS真空多联装置专门设计用于与MultiScreenHTS过滤板配合使用。这一组合提供了优化的过滤特征以及无缝自动化整合。

Millicell测定板
- 除了传统的测定板，过滤板还有配装细胞培养皿的型号，用于培养和测定悬浮的和粘附的细胞系。在滤板上生长的细胞比在传统塑料基质上生长的细胞分化更好，从而产生更多的体内样结果。该滤板/托盘组合可用于多种细胞测定，包括转运、毒性和筛选测定。
过滤器代码：IP

应用

研究类别
测定

外形

色码：透明

其他说明

自动化兼容：否

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

分析证书(COA)

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Marcelo Pires Amaral et al.
Vaccine, 38(20), 3653-3664 (2020-04-06)
The recent outbreaks of Zika virus (ZIKV) infection and the potential association with Guillain-Barré syndrome in adults and with congenital abnormalities have highlighted the urgency for an effective vaccine. The ZIKV Envelope glycoprotein (EZIKV) is the most abundant protein on
Thomas Schachtner et al.
Transplant international : official journal of the European Society for Organ Transplantation, 33(3), 288-297 (2019-11-07)
Preformed donor-reactive T cells are relatively resistant to standard immunosuppression and account for an increased incidence of T cell-mediated rejection (TCMR) and inferior kidney allograft outcomes. We analyzed 150 living donor kidney transplant recipients (KTRs) of a first kidney allograft.
Mara De Martino et al.
Oncoimmunology, 9(1), 1715767-1715767 (2020-02-18)
Stat3 is constitutively activated in several tumor types and plays an essential role in maintaining their malignant phenotype and immunosupression. To take advantage of the promising antitumor activity of Stat3 targeting, it is vital to understand the mechanism by which
Antje Blank et al.
NPJ vaccines, 5, 10-10 (2020-02-07)
A vaccine remains a priority in the global fight against malaria. Here, we report on a single-center, randomized, double-blind, placebo and adjuvant-controlled, dose escalation phase 1a safety and immunogenicity clinical trial of full-length Plasmodium falciparum merozoite surface protein 1 (MSP1)
Miriam Pedrera et al.
Vaccines, 8(1) (2020-03-07)
Nipah virus (NiV) is an emergent pathogen capable of causing acute respiratory illness and fatal encephalitis in pigs and humans. A high fatality rate and broad host tropism makes NiV a serious public and animal health concern. There is therefore

商品

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

实验方案

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

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