生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
C23.21, monoclonal
分子量
observed mol wt ~17 kDa
纯化方式
using protein G
种属反应性
all
包装
antibody small pack of 100 μL
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG1κ
表位序列
Unknown
UniProt登记号
运输
2-8°C
靶向翻译后修饰
unmodified
一般描述
Strep-Tag is a short amino acid-peptide sequence that exhibits specific binding to streptavidin. It is shown to reversibly occupy the same pocket where biotin is normally complexed. Hence, it is highly desirable for applications involving where efficient purification of fusion proteins. Use of this tag allows for easier detection of fusion proteins in various immunochemical assays. Strep-Tag is shown to be resistant to the action of proteases and can also be used in the presence of mild detergents. Clone C23.21 recognizes fusion proteins with Strep-Tag peptide sequence WSHPQFEK with picomolar affinity. It recognizes both the single and the double Strep-Tag sequence.
特异性
Clone C23.21 is a mouse monoclonal antibody that detects Strep-Tagged proteins. It binds to WSHPQFEK sequence with picomolar affinity.
免疫原
Recombinant ectodomain of glycoprotein E2 of GB virus (GBV-B), containing a double Strep-Tag (two Strep-Tags separated by a linker peptide)
应用
Quality Control Testing
Evaluated by Western Blotting with Strep-Tagged VHH 9.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Strep-Tagged VHH9.
Tested Applications
Surface plasmon resonance: A representative lot detected Strep-Tag in Surface plasmon resonance application. (Meola, A., et al. (2015). J Virol. 89(4):2170-81).
Western Blotting Analysis: A representative lot detected Strep-Tag in human brain extract and GFAP in Western Blotting applications. (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Immunohistochemistry Applications: A representative lot detected Strep-Tag in mouse brain tissue sections in Immunohistochemistry applications (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with Strep-Tagged VHH 9.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Strep-Tagged VHH9.
Tested Applications
Surface plasmon resonance: A representative lot detected Strep-Tag in Surface plasmon resonance application. (Meola, A., et al. (2015). J Virol. 89(4):2170-81).
Western Blotting Analysis: A representative lot detected Strep-Tag in human brain extract and GFAP in Western Blotting applications. (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Immunohistochemistry Applications: A representative lot detected Strep-Tag in mouse brain tissue sections in Immunohistochemistry applications (Li, T., et al. (2017). Immunol Lett. 188:89-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Strep-Tag, clone C23.21, Cat. No. MAC143, is a mouse monoclonal antibody that detects Strep-Tagged proteins and is tested for use in Immunohistochemistry, Surface plasmon resonance, and Western Blotting.
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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