推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
2F5, monoclonal
种属反应性
human, rat
技术
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
human ... GJA1(2697)
rat ... Gja1(24392)
一般描述
Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.
特异性
Clone 2F5 detects connexin-43 in human and rat heart. It targets an epitope within 136 amino acids from the C-terminal half.
免疫原
A recombinant fragment corresponding to 136 amino acids from the C-terminal half of Rat connexin-43.
Epitope: C-terminus
应用
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Connexin 43 in rat heart tissue.
Western Blotting Analysis: A representative lot detected Connexin 43 in mouse heart tissue lysates, NRK cell lysates, and MDCK cell lysates (Courtesy of Joell Solan in Paul Lampe′s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).
Immunofluorescence Analysis: A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe′s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).
Western Blotting Analysis: A representative lot detected Connexin 43 in mouse heart tissue lysates, NRK cell lysates, and MDCK cell lysates (Courtesy of Joell Solan in Paul Lampe′s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).
Immunofluorescence Analysis: A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe′s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).
Research Category
Cell Structure
Cell Structure
This mouse monoclonal Anti-Connexin 43, clone 2F5, Cat. No. MABT917 is tested for use in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting for the detection of Connexin-43.
质量
Evaluated by Immunohistochemistry in human heart tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Connexin 43 in human heart tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Connexin 43 in human heart tissue.
目标描述
43.03 kDa calculated.
外形
Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
法规信息
新产品
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门