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主要文件

MABT844

Sigma-Aldrich

Anti-Cytokeratin 8/18 Antibody, clone L2A1

clone L2A1, from mouse

别名:

Cytokeratin-8/18, Keratin type II cytoskeletal 8/Keratin type I cytoskeletal 18, Keratin-8/Keratin-18, K8/K18)

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250 MG
¥612.32
1 G
¥997.71

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250 MG
¥612.32
1 G
¥997.71

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.43

¥612.32


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生物来源

mouse

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

L2A1, monoclonal

种属反应性

human

包装

antibody small pack of 25 μg

技术

immunocytochemistry: suitable
immunoprecipitation (IP): suitable

同位素/亚型

IgGκ

UniProt登记号

靶向翻译后修饰

unmodified

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MABT875WH0003875M1SAB1306569
biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, mouse

clone

L2A1, monoclonal

clone

IB4, monoclonal

clone

2F8, monoclonal

clone

polyclonal

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL

technique(s)

flow cytometry: 1:10-1:50, immunofluorescence: 1:10-1:50, immunohistochemistry: 1:10-1:50, western blot: 1:1000

一般描述

Cytokeratin-8/18 (UniProt: P05787/P05783; also known as Keratin, type II cytoskeletal 8/ Keratin, type I cytoskeletal 18, Keratin-8/Keratin-18, K8/K18) is encoded by the KRT8/KRT18 (also known as CYK8/CYK18) gene (Gene ID: 3856/3875) in human. Cytokeratins 8 and 18 (K8/18) are simple epithelial cell-specific intermediate filament proteins. Cytokeratin-8 is a type II, neutral to basic, protein of the intermediate filament family that together with Cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is shown to be highly conserved in all type II keratins. Phosphorylation at Serine 73 is reported to increase during cellular stress, including hear and drug exposure. However, under normal conditions serine 73 remains largely dephosphorylated. Cytokeratin-8 can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. Cytokeratin-18 is a type I, acidic, protein that is expressed in colon, placenta, and liver. Higher expression levels have been reported in lymph nodes of breast carcinoma. Cytokeratin-18 is involved in the uptake of thrombin-antithrombin complexes by hepatic cells. Upon phosphorylation, it plays a role in filament reorganization. It is proteolytically cleaved by caspases during epithelial cell apoptosis and this cleavage is shown to occur at Asp-238. Phosphorylation of cytokeratin-18 at serine 33 is shown to increase during mitosis in cultured cells and in regenerating liver. Serine 33 phosphorylation is considered to be essential for its association with 14-3-3 proteins and has a role in keratin organization and distribution. Cytokeratin-18 can undergo O-glycosylation, which results in its increased solubility and reduction in its stability. Mutations in KRT8 and KRT18 genes have been been linked to liver cirrhosis that is characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper.

特异性

Clone L2A1 is a mouse monoclonal antibody that detects human cytokeratin 8/18.

免疫原

KLH-conjugated Linear Peptide corresponding to a sequence from Human Cytokeratin 8/18.

应用

Anti-Cytokeratin 8/18, clone L2A1, Cat. No. MABT844, is a mouse monoclonal antibody that detects cytokeratin 8/18 and has been tested for use in Immunocytochemistry and Immunoprecipitation.
Immunoprecipitation Analysis: A representative lot detected Cytokeratin 8/18 in Immunoprecipitation applications (Haltiwanger, R.S., et. al. (1997). J Biol Chem. 272(13):8752-8; Chou, C.F., et. al. (1994). Biochem J. 298 ( Pt 2):457-63; Chou, C.F., et. al. (1993). Biochem J. 268(6):4465-72; Ku, N.O., et. al. (2000). J Cell Biol. 149(3):547-52; Lahdeniemi, I.A.K., et. al. (2017). Cell Death Differ. 24(6):984-996).

Immunocytochemistry Analysis: A representative lot detected Cytokeratin 8/18 in Immunocytochemistry applications (Chou, C.F., et. al. (1993). Biochem J. 268(6):4465-72).
Research Category
Cell Structure

质量

Evaluated by Immunocytochemistry in HT-29 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Cytokeratin 8/18 in HT-29 cells.

目标描述

~53.70 kDa and 48.06 kDa, respectively for cytokeratin 8 and 18 calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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