Anti-ADAM 17 Antibody, clone 16C11.1 | MABT388

Disintegrin and metalloproteinase domain-containing protein 17 (EC 3.4.24.86; UniProt P78536; also known as ADAM 17, CD156b, Snake venom-like protease. TNF-alpha convertase, TNF-alpha-converting enzyme, Tumor necrosis factor alpha-converting enzyme) is encoded by the ADAM17 (also known as CD156B, CSVP, NISBD, NISBD1, TACE) gene (Gene ID 6868) in human. Originally described as a TNF–alpha converting enzyme, ADAM17 is known to process the cleavage of many transmembrane proteins, including membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 was the first ′sheddase′ identified. ADAM17 activity is regulated by multiple post-translational modifications. It has three phosphorylation sites on its C-terminal cytoplasmic domain (T735, S791, and (S819). ERK1/2-mediated T735 phosphorylation is reported to regulate ADAM17 maturation and surface expression. ADAM 17 is initially translated in its prepro-form and subsequent cleavages of the signal (aa 1-17) and propeptide (18-214) sequences produces the 610 aa mature ADAM 17 (aa 215-824).

Expected to react with both spliced isoforms.

GST-tagged recombinant protein corresponding to human ADAM 17.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This mouse Monoclonal Anti-ADAM 17 Antibody, clone 16C11.1, Cat. No. MABT388 detects levels of ADAM17, and has been published and validated for use in Western Blotting, Immunohistochemistry (Paraffin).

Western Blotting Analysis: 5.0 µg/mL from a representative lot detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ADAM 17 in human testis and colorectal cancer tissues.

Evaluated by Western Blotting in human acute myelogenous leukemia KG-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.

~120 kDa observed. A lower band ~100 kDa is also observed when using a higher antibody concentration (5 µg/mL). Due to glycosylation, target band size appears larger than the calculated molecular weights (93.0 kDa/prepro-isoform A, 68.2 kDa/mature isoform A, 78.5kDa/prepro-isoform B, 63.7 kDa/mature isoform B). Uncharacterized band(s) may appear in some lysates.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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