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MABT334

Sigma-Aldrich

Anti-WASF2/WAVE-2 Antibody, clone ZZ36-12

clone ZZ36-12, from mouse, purified by affinity chromatography

别名:

WASF2, WAVE-2, Wiskott-Aldrich syndrome protein family member 2, WASP family protein member 2, Protein WAVE-2, Verprolin homology domain-containing protein 2

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

ZZ36-12, monoclonal

纯化方式

affinity chromatography

种属反应性

human

技术

immunocytochemistry: suitable
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... WASF2(10163)

一般描述

Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WASF2/WAVE-2) has a very wide distribution with strong expression in peripheral blood leukocytes. WASF2/WAVE-2 is involved in signaling RTKs and small GTPases. In general WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.

免疫原

Epitope: C-terminus
His-tagged recombinant protein corresponding to the C-terminus of Human WASF2/WAVE-2.

应用

Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-WASF2/WAVE-2 Antibody, clone ZZ36-12 is validated for use in Western Blotting and Immunocytochemistry for the detection of WASF2/WAVE-2.
Western Blotting Analysis: 1 µg/mL of a representative lot of this antibody detected WASF2/WAVE-2 in 10 µg of K562 cell lysate.

Western Blotting Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cell lysate +/- RNAi.

Immunocytochemistry Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cells.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected WASF2/WAVE-2 in 10 µg of HeLa cell lysate.

目标描述

~65 kDa observed.
May run at ~65-80 kDa, which is higher than calculated molecular weight of 54 kDa, due to the proline rich residues in the sequence (Yan, C., et al. (2003) EMBO J. 22(14): 3602–3612).

外形

Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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