生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
24-21, monoclonal
种属反应性
human
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ABI1(10006)
一般描述
c-Abl, v-Abl, and Bcr-abl are ubiquitously expressed non-receptor tyrosine kinases that are involved in a plethora of cellular processes. c-Abl is involved in cytoskeleton remodeling and facilitates cell adhesion and cell motility. c-Abl achieves cytoskeleton remodeling by phosphorylating and activating a wide variety of cytoskeleton-associated proteins such as WASF3, ANXA1, DBN1, DBNL, CTTN, RAPH1, ENAH, MAPT, and PXN. Abl-1 also phosphorylates a number of receptor tyrosine kinases and is involved in regulating levels of EGFR by endocytosis. c-Abl may also play a role in DNA damage response via the ATM pathway, among other cellular processes. Whereas v-Abl is known to be involved in pre-B cell transformation in mice, Bcr-Abl is reported to promote cellular growth in leukemia cells and maintain CML phenotype. Many v-Abl- and Bcr-abl-mediated signaling events have been described; however the complexity and function of these pathways require further elucidation.
特异性
This antibody recognizes the c-terminus of c-Abl, v-Abl, and Bcr-Abl.
免疫原
Recombinant protein corresponding to the carboxyl region of human v-Abl fused with TrpE.
应用
Anti-Abl (c-, v-, Bcr-) Antibody, clone 24-21 detects level of Abl (c-, v-, Bcr-), clone 24-21 & has been published & validated for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Abl (c-, v-, Bcr-) in COS-7 and 7C411 cells (Goga, A., et al. (1993). Mol Cell Biol. 13(8):4967-49775.).
质量
Evaluated by Western Blot in K562 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Abl (c-, v-, Bcr-) in 10 µg of K562 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Abl (c-, v-, Bcr-) in 10 µg of K562 cell lysate.
目标描述
~125 kDa and ~190 kDa observed. Uniprot describes a molecular weight of c-Abl at ~122 kDa Uniprot describes that this protein is subject to post-translational modification. This antibody detected c-Abl (~125 kDa) and Bcr-Abl (~190 kDa) in K562 cell lysate. An uncharacterized band at ~27 kDa may be observed in some cell lysates.
外形
Format: Purified
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
F Belloc et al.
Cell death and differentiation, 4(8), 806-814 (2006-02-09)
Apoptosis was studied in parental and mdr-1 expressing U937, HL60 and K562 myeloid leukemic cell lines using mdr unrelated inducers of apoptosis such as Ara-C, cycloheximide, serum deprivation, ceramide, monensin and UV irradiation. Apoptosis was efficiently induced by all these
Abelson interactor 1 (ABI1) and its interaction with Wiskott-Aldrich syndrome protein (wasp) are critical for proper eye formation in Xenopus embryos.
Singh, A; Winterbottom, EF; Ji, YJ; Hwang, YS; Daar, IO
The Journal of Biological Chemistry null
Giovanni Amabile et al.
Nature communications, 6, 7091-7091 (2015-05-23)
Chronic myeloid leukaemia (CML) is a myeloproliferative disorder characterized by the genetic translocation t(9;22)(q34;q11.2) encoding for the BCR-ABL fusion oncogene. However, many molecular mechanisms of the disease progression still remain poorly understood. A growing body of evidence suggests that the
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