Anti-Cadherin 6 Antibody, clone 2B6-D8

Cadherin-6 (UniProt: P55285; also known as Kidney cadherin, K-cadherin) is encoded by the CDH6 gene (Gene ID: 1004) in human. Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin-6 is a class II cadherin that is highly expressed in brain and kidney. Its lower expression is also reported in lung, pancreas, and gastric mucosa. It is involved in the morphogenesis of central nervous system and kidney. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18) and a propeptide (aa 19-53) that are subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 54-615), a transmembrane domain (aa 616-636), and a cytoplasmic domain (aa 637-790). It has 5 cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections and thereby imparting a strong curvature to the full-length ectodomain. Cadherin-6 is reported to drive EMT during embryonic development. It acts as a metastasis suppressor molecule and its expression is significantly reduced with an increase in tumor grade and stage in renal cell carcinoma. (Ref.: Gugnoni, M., et al. (2017). Oncogene 36(5); 667-677; Shimazui, T., et al. (1998). Clin. Cancer Res. 4(10); 2419-2424).

Clone 2B6-D8 is a mouse monoclonal antibody that detects human Cadherin-6. It targets an epitope within the extracellular domain.

GST-tagged recombinant protein fragment corresponding to the extracellular domain of human Cadherin-6.

Quality Control Testing

Evaluated by Western Blotting in Human kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cadherin-6 in Human kidney tissue lysate.

Tested Applicaitions

Western Blotting Analysis: A representative lot detected Cadherin-6 in Western Blotting applications (Shimazui, T., et al. (1998). Clin Cancer Res. 4(10); 2419-24).

Immunohistochemistry Applications: A representative lot detected Cadherin-6 in Immunohistochemistry applications (Shimazui, T., et al. (1998). Clin Cancer Res. 4(10); 2419-24).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Cadherin 6, clone 2B6-D8, Cat. No. MABT1579, is a mouse monoclonal antibody that detects Cadherin-6 and is tested for use in Immunohistochemistry and Western Blotting.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.