Anti-Profilin IIa Antibody, clone PF2A1-8F8

Profilin-2 (UniProt: P35080; also known as Profilin II) is encoded by the PFN2 gene (Gene ID: 5217) in human. Profilins are actin-binding proteins that serve as key regulators of actin polymerization in cells. Five profilin isoforms have been identified in mammals: Profilins I, IIa, IIb, III and IV. Profilin binds to G-actin in a 1:1 complex and thereby sequesters monomeric actin. Profilin I is highly expressed in all tissues except skeletal muscle, heart, and brain, while profilin II is highly expressed in brain, skeletal muscle, and kidney. Profilin IIa is highly expressed in developing and adult neurons and is involved in correct neurite outgrowth. Profilin IIb appears to be a minor form and it differs from IIa in 13 amino acids in the C-terminal region. Knockdown of both profilins I and II is reported to inhibit neurite outgrowth, possibly impairing actin dynamics by reducing the availability of profilin -G-actin complexes for microfilament growth. In addition to its role in actin polymerization and neurite outgrowth, profilin IIa may also be involved in regulation of synaptic vesicles. It exhibits high affinity for proline-rich peptides and is shown to interact with survival of motor neurons protein (SMN). Mutated forms of SMN (Y272G and G279V) are reported to significantly reduce its interaction with profilin IIa. (Ref.: Sharma, A., et al. (2005). Exp. Cell Res. 305; 195-197; Witke, W., et al. (1998). EMBO J. 17; 967-976).

Clone PF2A1-8F8 is a mouse monoclonal antibody that specifically detects Profilin-II. It targets an epitope within 10 amino acids from the C-terminal half.

T7-tagged full-length recombinant human Profilin IIa.

Quality Control Testing

Evaluated by Western Blotting in Human cerebral cortex tissue lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Profilin IIa in Human cerebral cortex tissue lysate.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated Profilin IIa in Immunoprecipitation applications (Sharma, A., et al. (2005). Exp Cell Res. 309(1): 185-97).

Immunofluorescence Analysis: A representative lot detected Profilin IIa in Immunofluorescence applications (Sharma, A., et al. (2005). Exp Cell Res. 309(1): 185-97).

Western Blotting Analysis: A representative lot detected Profilin IIa in Western Blotting applications (Sharma, A., et al. (2005). Exp Cell Res. 309(1): 185-97).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Profilin IIa, clone PF2A1-8F8, Cat. No. MABT1574, is a mouse monoclonal antibody that detects Profilin IIa and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.