Anti-SNX9 Antibody, clone 6C6

Sorting nexin-9 (UniProt: Q9Y5X1; also known as SH3 and PX domain-containing protein 1, Protein SDP1, SH3 and PX domain-containing protein 3A, SNX9) is encoded by the SNX9 (also known as SH3PX1, SH3PXD3A) gene (Gene ID: 51429) in human. SNX9 is widely expressed, with highest levels reported in heart and placenta, and lowest levels in thymus and peripheral blood leukocytes. SNX9 is localized at sites of endocytosis at the cell membrane and is detected on newly formed macropinosomes. It is transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. SNX9 can exist as a homo dimer or homo oligomer and can also heterodimerize with SNX18. It displays four phosphatidylinositol 4,5-bisphosphate binding sites. It has a SH3 domain (aa 1-63), a SNX-type Phox homology (PX) domain (aa 250-361), and a BAR domain (aa 292-595). The PX domain mediates its interaction with membranes enriched in phosphatidylinositol phosphate. SNX9 can stimulate the GTPase activity of dynamin and both its SH3 domain and more C-terminal domains are required to efficiently stimulate this GTPase activity. Although it has high affinity for phosphatidylinositol 4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates. SNX9 can be phosphorylated on tyrosine residues by TNK2 and phosphorylation promotes its activity in the degradation of EGFR. SNX9 is shown to be involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. It is required for efficient progress through mitosis and cytokinesis and also for normal formation of the cleavage furrow at the end of mitosis. (Ref.: Schobel, S., et al. (2008). J. Biol. Chem.283(21); 14257-14268).

Clone 6C6 is a rat monoclonal antibody that detects human Sorting nexin 9 (SNX9). It targets an epitope with in the SH3 domain.

GST-tagged full-length recombinant human Sorting Nexin-9 protein.

Anti-SNX9, clone 6C6, Cat. No. MABT1538, is a rat monoclonal antibody that detects human Sorting nexin-9 protein and has been tested for use in Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: A representative lot detected SNX9 in Western Blotting applications (Schobel, S., et. al. (2008). J Biol Chem. 283(21):14257-68).

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected SNX9 in HCT116 cell lysate.

~74 kDa observed; 66.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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