Anti-Alpha-Catenin Antibody, clone 1G5

Catenin alpha 1 (UniProt: E1C7M7) is encoded by the CTNNA1 gene in chicken. Catenins are a large family of cell-cell adhesion molecules that are involved in development and in epithelial cell polarity. Catenins are reported to associate with both E- and N-cadherins. The association of catenins to cadherins creates a complex that is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Alpha-Catenin is considered as an important regulator of actin dynamics in cell-cell adhesion and it forms a heterodimer with beta-Catenin and links the cadherins to actin. Unlike other catenins, alpha-catenin does not bind to cadherins directly, but is linked to the adhesive complexes of AJs via association with -catenin. Alpha-catenin can also bind and bundle actin filaments and can interact with a number of protein regulators of actin filament turnover. Usually cells contain a significant amount of cadherin-free alpha-catenin and this pool of free alpha-catenin can impact actin dynamics, such as limiting Arp2/3-based actin polymerization and severing by cofilin that ultimately promotes the assembly and stabilization of unbranched filament bundles. Alpha-catenin expression in a colon cancer cell lines has been correlated with increased Tcf-dependent transcription and is shown to inhibit beta-catenin signaling in the nucleus by interfering with the formation of a beta-catenin-Tcf-DNA complex. (Ref.: Giannini, AL et al. (2000). J. Bol. Chem. 275(29); 21883-8).

Clone 1G5 specifically detects alpha-catenin 1 in human and chicken cells.

MBP-conjugated full-length chicken recombinant Catenin alpha 1.

Anti-Alpha-Catenin, clone 1G5, Cat. No. MABT1508, is a mouse monoclonal antibody that detects Catenin alpha 1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Alpha-Catenin in Immunoprecipitation applications (Knudsen, K.A., et. al. (1995). J Cell Biol. 130(1):67-77; Johnson, K.R., et. al. (1993). Exp Cell Res. 207(2):252-60).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Alpha-Catenin in A431 cell line.

Western Blotting Analysis: A representative lot detected Alpha-Catenin in Western Blotting applications (Nieman, M.T., et. al. (1999). J Cell Sci. 112 ( Pt 10):1621-32; Knudsen, K.A., et. al. (1995). J Cell Biol. 130(1):67-77; Johnson, K.R., et. al. (1993). Exp Cell Res. 207(2):252-60).

Immunocytochemistry Analysis: A representative lot detected Alpha-Catenin in Immunocytochemistry applications (Johnson, K.R., et. al. (1993). Exp Cell Res. 207(2):252-60).

Research Category
Cell Structure

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Alpha-Catenin in A431 cell lysate.

~108 kDa observed; 59.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.