Anti-JAM-A Antibody, clone BV11

Junctional adhesion molecule A (JAM-A) is found in endothelial and epithelial tight junctions and is involved in the inflammatory enlistment of mononuclear cells containing LFA-1. JAM-A is linked to atherogenesis, platelet activation, transmigration of leukocytes and angiogenesis. JAM-A is also found to be important in the development of many different organ systems, and is mostly expressed in the epithelial parts and the embryonic vasculature. JAM-A is indicated to play a role in epithelial permeability, inflammation and proliferation in the intestine, aiding in homeostasis.

H5V murine heart capillary endothelial cells.

Detect JAM-A using this Anti-JAM-A Antibody, clone BV11 validated for use in FC, IC & IP.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in cell extracts from Mouse H5V and PDV cells, and in COS cultures transfected with full length cDNA of mouse JAM-A (Martin-Padura, I., et al. (1998). J Cell Biol. 142(1):117-127).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in cultured epithelial cells, cremaster muscles, and Schwann Cells (Martin-Padura, I., et al. (1998). J Cell Biol. 142(1):117-127).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Evaluated by Flow Cytometry in bEnd.3 cells.

Flow Cytometry Analysis: A 1:1,000 dilution of this antibody detected JAM-A in bEnd.3 cells.

32 kDa calculated

Protein G Purified

Format: Purified

Purified rat monocolonal IgG2bκ in buffer containing PBS and no preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
bEnd.3 cells

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