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Merck
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主要文件

安全信息

MABS417

Sigma-Aldrich

Anti-GOT2, clone 3E9 Antibody

ascites fluid, clone 3E9, from mouse

别名:

Aspartate aminotransferase, mitochondrial, mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine-oxoglutarate transaminase 4, Kynurenine--oxoglutarate

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

3E9, monoclonal

种属反应性

mouse, rat, monkey, human

技术

immunofluorescence: suitable
western blot: suitable

同位素/亚型

IgG1

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... GOT2(2806)

一般描述

Aspartate aminotransferase, mitochondrial ((mAspAT), also known as Fatty acid-binding protein (FABP-1) or Glutamate oxaloacetate transaminase 2 or Kynurenine aminotransferase 4 or Kynurenine aminotransferase IV or Kynurenine--oxoglutarate transaminase 4 or Kynurenine--oxoglutarate transaminase IV or Plasma membrane-associated fatty acid-binding protein (FABPpm) or Transaminase A, and encoded by the gene name GOT2, plays an essential role in role in amino acid metabolism and facilitates cellular uptake of long-chain free fatty acids. GOT2 is localized to the mitochondrion; however, exposure to alcohol promotes translocation to the cell membrane.

免疫原

Purified recombinant fragment of human GOT2 expressed in E. Coli

应用

Anti-GOT2, clone 3E9 Antibody is an antibody against GOT2 for use in western blotting & immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GOT2 in PC-3 and SK-BR-3 cells.
Research Category
Signaling
Research Sub Category
Metabolic Hormones & Receptors

质量

Evaluated by Western Blotting in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GOT2 in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.

目标描述

~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Jing Xu et al.
Translational stroke research, 11(3), 418-432 (2019-09-02)
The preservation of mitochondrial function is a major protective strategy for cerebral ischemic injuries. Previously, our laboratory demonstrated that protein kinase C epsilon (PKCε) promotes the synthesis of mitochondrial nicotinamide adenine dinucleotide (NAD+). NAD+ along with its reducing equivalent, NADH

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