Anti-SFRP2 Antibody, clone 8H1

Secreted frizzled-related proteins (SFRPs) may play a critical role in carcinogenesis by inhibiting the Wnt/beta-catenin signaling pathway. Secreted frizzled-related protein 2 (SFRP2) may be useful to further investigate mammary tumorigenesis as its expression increases with tumor progression in canine mammary gland tumors. SFRP2 in mouse embryos has been found to cause severe shortening of the thoracic region. Studies have shown that this protein is the essential stem cell paracrine factor that is thought to regulate myocardial survival and repair damage in the aftermath of an ischemic injury.

Recombinant protein corresponding to human SFRP2.

Detect SFRP using this rat monoclonal antibody, Anti-SFRP2 Antibody, clone 8H1 validated for use in western blotting & IHC.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A representative lot from an independent laboratory detected SFRP2 in RMG cell lysate and in ERU retinal tissue lysate (Hauck, S. M., et al. (2012). Journal of Proteomics. 75:4545-4554).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SFRP2 in equine retinal tissue (Hauck, S. M., et al. (2012). Journal of Proteomics. 75:4545-4554).

Evaluated by Western Blotting in mouse eye tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SFRP2 in 10 µg of mouse eye tissue lysate.

~ 33 kDa observed

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse eye tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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