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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2G10, monoclonal
分子量
calculated mol wt 139.51 kDa
observed mol wt ~150 kDa
纯化方式
using protein G
种属反应性
human
种属反应性(根据同源性预测)
hamster
包装
antibody small pack of 100 μL
技术
western blot: suitable
同位素/亚型
IgG1κ
表位序列
N-terminal half
Protein ID登记号
UniProt登记号
运输
2-8°C
靶向翻译后修饰
unmodified
基因信息
hamster ... SCAP(100689048)
一般描述
Sterol regulatory element-binding protein cleavage-activating protein (UniProt P97260; also known as SCAP, SREBP cleavage-activating protein) is encoded by the SCAP gene (Gene ID 100689048) in hamster species. SCAP is a multi-pass membrane protein with five cytoplasmic domains, eight transmembrane domains, and four lumenal domains. The C-terminal domain of SCAP mediates association with SREBPs, while transmembrane helices 2 6 comprise the sterol-sensing domain that mediates sterol-induced binding of SCAP to Insigs. SCAP is mainly localized in endoplasmic reticulum and Golgi apparatus. It requires the presence of SPRING for proper localization to endoplasmic reticulum. It mediates the activation of membrane-bound transcription factors known as sterol-regulatory element binding proteins (SREBPs) for transcribing genes encoding cholesterol biosynthetic enzymes. Low sterol concentrations are reported to trigger release of Insig and lead to a conformational change in the SSD domain (aa 284-442) of SCAP, unmasking of the ER export signal and promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi. In the Golgi, SREBPs are proteolytically processed releasing the transcription factor fragment of SREBPs from the membrane. At high sterol concentrations, formation of a ternary complex with Insig leads to masking of the ER export signal in SCAP, which promotes retention of the complex in the endoplasmic reticulum. (Ref.: Kober, DL., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(45); 28080-28091).
特异性
Clone 2G10 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein cleavage-activating protein (SACP).
免疫原
Recombinant fragment corresponding to the first 767 amino acids of the Chinese Hamster SCAP protein combined with Ribi adjuvant.
应用
Quality Control Testing
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected SCAP in PC3 cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected SCAP in Western Blotting applications (Kober D.L., et al. (2020). Proc Natl Acad Sci USA. 117(45):28080-28091).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected SCAP in PC3 cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected SCAP in Western Blotting applications (Kober D.L., et al. (2020). Proc Natl Acad Sci USA. 117(45):28080-28091).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SCAP, clone 2G10, Cat. No. MABS2288, is a mouse monoclonal antibody that SREBP cleavage-activating protein (SCAP) and is tested for use in Western Blotting.
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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