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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
8G1, monoclonal
分子量
calculated mol wt 504.61 kDa
纯化方式
using protein G
种属反应性
human
包装
antibody small pack of 100 μg
技术
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
表位序列
N-terminal
Protein ID登记号
UniProt登记号
运输
2-8°C
靶向翻译后修饰
unmodified
一般描述
Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APER, Apolipoprotein E receptor, CD19. LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is single pass type I membrane protein that is expressed in most tissues, but is abundant in liver, brain, and lung. It serves as a major VSMC receptor with a crucial role in maintaining vessel wall integrity and its expression in adult smooth muscle cells is shown to be essential for maintaining a contractile phenotype. LRP1 is synthesized with a signal peptide sequence (aa 1-19) and is processed in trans-Golgi network by Furin to generate a 515 kDa a subunit and an 85 kDa b subunit that contains the 100-amino intracellular domain (LRPICD; aa 4445-4544). The a and b subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid b. It is the primary receptor mediating transport of amyloid b peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (a2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP1 mediated clearance of amyloid b peptides across the blood brain barrier, which may contribute to complications of Alzheimer′s disease.
特异性
Clone 8G1 is a mouse monoclonal antibody that detects human LRP-1. It targets an epitope within the a chain in the extracellular domain.
免疫原
Full-length LRP-1 purified from human placenta and 515 kDa band excised from gel and used as immunogen.
应用
Quality Control Testing
Evaluated by Immunocytochemistry in MCF-7 cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LRP1 a-chain in MCF-7 cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected LRP1 -chain in Immunocytochemistry applications (Craig, J., et al. (2013). PLoS One. 8(7):e70432).
Immunoprecipitation Analysis: A representative lot immunoprecipitated LRP1 -chain in Immunoprecipitation applications (Ranganathan, S., et al. (2011). J. Biol. Chem.286(35):30535-30541; Boye, K., et al. (2017). Nat. Commun. 8(1):1571).
Western Blotting Analysis: A representative lot detected LRP1 -chain in Western Blotting applications (Strickland, D.K., et al. (1990). J Biol Chem.;265(29):17401-4; Yamamoto, K., et al. (2013). FASEB J.;27(2):511-21; Yamamoto, K. et al. (2016). Matrix Biol.;56:57-73; Boye, K., et al. (2017). Nat Commun.;8(1):1571).
ELISA Analysis: A representative lot detected LRP1 -chain in ELISA applications (Ranganathan, S., et al. (2011). J. Biol. Chem. 286(35):30535-3054).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunocytochemistry in MCF-7 cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LRP1 a-chain in MCF-7 cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected LRP1 -chain in Immunocytochemistry applications (Craig, J., et al. (2013). PLoS One. 8(7):e70432).
Immunoprecipitation Analysis: A representative lot immunoprecipitated LRP1 -chain in Immunoprecipitation applications (Ranganathan, S., et al. (2011). J. Biol. Chem.286(35):30535-30541; Boye, K., et al. (2017). Nat. Commun. 8(1):1571).
Western Blotting Analysis: A representative lot detected LRP1 -chain in Western Blotting applications (Strickland, D.K., et al. (1990). J Biol Chem.;265(29):17401-4; Yamamoto, K., et al. (2013). FASEB J.;27(2):511-21; Yamamoto, K. et al. (2016). Matrix Biol.;56:57-73; Boye, K., et al. (2017). Nat Commun.;8(1):1571).
ELISA Analysis: A representative lot detected LRP1 -chain in ELISA applications (Ranganathan, S., et al. (2011). J. Biol. Chem. 286(35):30535-3054).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LRP1 a-chain, clone 8G1, Cat. No. MABS2253, is a mouse monoclonal antibody that detects LRP1 a -chain and is tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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