Anti-ST8SIA1 Antibody, clone HIC0-3C5

Evaluated by Immunohistochemistry in human pancreatic tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected ST8SIA1 in human pancreatic tissue sections.

Anti-ST8SIA1, clone HIC0-3C5, Cat. No. MABS1997, is a mouse monoclonal antibody that detects Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase and has been tested for use in Flow Cytometry, Immunoflurescence, and Immunohistochemistry.

Flow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).

Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).

Research Category
Signaling

Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

40.52 kDa calculated.

Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (UniProt: Q92185; also known as EC: 2.4.99.8, Alpha-2,8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI) I encoded by the ST8SIA1 (also known as SIAT8, SIAT8A) gene (Gene ID: 6489) in human. SIAT8-A is a single-pass type II membrane protein that is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. It is involved in the production of gangliosides GD3 and GT3 from GM3. It catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3 in a successive manner. It is also involved in the pathway protein glycosylation. SIAT8-A contains a cytoplasmic domain (aa 1-29); a transmembrane domain (aa 30-48); and a lumenal domain (aa 49-356). Two isoforms of SIAT8-A have been described that are produced by alternative splicing. (Ref.: Dorrell, C., et al. (2016). Nat. Commun. 7; 11756).

Trypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.

Concentration: Please refer to lot specific datasheet.

Format: Purified

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.