Anti-TC PTPase (PTPN2) Antibody, clone 9E9.1

Tyrosine-protein phosphatase non-receptor type 2 (UniProt: P17706; also known as EC: 3.1.3.48, T-cell protein-tyrosine phosphatase, TCPTP) is encoded by the PTPN2 (also known as PTPT) gene (Gene ID: 5771) in human. TCPTP is a non-receptor type tyrosine-specific phosphatase that dephosphorylates a variety of receptor protein tyrosine kinases. It is also reported to dephosphorylate non-receptor tyrosine kinases, such as JAK 1, 2, 3, Src kinases, STAT1, 3, 5, 5B, and 6. TCPTP is ubiquitously expressed and two isoforms have been reported. Isoform 1 is expressed in T-cells and in placenta and is targeted to the endoplasmic reticulum by its C-terminal hydrophobic region. Isoform 2 is reported to be the major isoform that predominantly localizes to chromatin and is able to shuttle between the nucleus and the cytoplasm and to plasma membrane to dephosphorylate receptors. It doing so it negatively regulates numerous signaling pathways and biological processes, including hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. It is also reported to play a multifaceted and important role in the development of the immune system. In T-cell receptor signaling, it functions through dephosphorylation of FYN and LCK to control T-cell differentiation and activation. It also dephosphorylates CSF1R and thereby negatively regulates its downstream signaling and macrophage differentiation.

Clone 9E9.1 detects human T-cell protein-tyrosine phosphatase. It targets an epitope within 79 amino acids from the C-terminal half.

GST/His-tagged recombinant fragment corresponding to 79 amino acids from the C-terminal half of human T-cell protein-tyrosine phosphatase.

Anti-TC PTPase (PTPN2), clone 9E9.1, Cat. No. MABS1753, is a mouse monoclonal antibody that detects T-cell protein-tyrosine phosphatase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TC PTPase (PTPN2) in human liver, human tonsil, and human cervical cancer tissues.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected TC PTPase (PTPN2) in 10 µg of Jurkat cell lysate.

Research Category
Signaling

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TC PTPase (PTPN2) in 10 µg of K562 cell lysate.

~48 KDa observed; 48.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.