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Merck
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MABN866

抗-肌纤蛋白（NT）抗体，克隆7.1

clone 7.1, from mouse

别名:

Myocilin, Myocilin 55 kDa subunit, Trabecular meshwork-induced glucocorticoid response protein, Myocilin, N-terminal fragment, Myocilin 20 kDa N-terminal fragment

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗-肌纤蛋白（NT）抗体，克隆7.1, clone 7.1, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7.1, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_000252

UniProt accession no.

Q99972

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MYOC(4653)

相关类别

Analysis Note

通过蛋白质印迹在小鼠眼组织裂解物中进行评价。

蛋白质印迹分析：0.5 µg/mL的该抗体在10 µg小鼠眼组织裂解物中检测到Myocilin。

Application

免疫组织化学分析：一个代表性批次在人小梁组织中检测到Myocilin（Ezzat，M.K.，et al.（2008）.Exp.Eye Res. 87（4）:376-384）。
蛋白质印迹分析：一个代表性批次在人小梁组织中检测到Myocilin（Ezzat，M.K.，et al.（2008）.Exp.Eye Res. 87（4）:376-384）。
蛋白质印迹分析：一个代表性批次在眼房水中检测到了Myocilin（Zhao，Y.，et al.（2013）.Mol.Cell Biol. 33（21）:4225-4240）。
抗Myocilin抗体，克隆7.1是抗Myocilin的抗体，用于蛋白质印迹、免疫组织化学。
研究子类别
发育信号传导
研究类别
神经科学

Disclaimer

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

General description

Myocilin（突变的小梁网诱导的糖皮质激素反应蛋白）由人的MYOC基因（也称为GLC1A、GPOA、JOAG、JOAG1、TIGR）编码（Entrez基因ID 4653）。Myocilin以504个氨基酸的糖蛋白形式产生，其中包含一个N末端疏水信号肽序列、一个卷曲螺旋亮氨酸拉链结构域和一个与嗅觉素具有同源性的C末端结构域。Myocilin通常通过凝胶电泳鉴定为53/57 kDa两条带，并且还报道了66 kDa形式的蛋白质。已知Myocilin在Glu214和Leu215之间进行细胞内蛋白水解切割，从而产生20 kDa的N末端和35 kDa的C末端片段。Myocilin被确定为Lingo-1受体配体，MYOC基因突变与10%的青少年开角型青光眼病例和3-4%的原发性开角型青光眼病例相关（PMID 24732711 24741044，24837143）。目录号MABN866，克隆7.1是一种小鼠单克隆抗体，可识别N末端片段（aa33-214）内的表位，并被证明适用于蛋白质印迹、免疫细胞化学和免疫组织化学应用。该克隆对人和鼠物种均具有反应性。（PMID 18674535 & 23979599）。
观测分子量~53、60 kDa

Immunogen

对应于人Myocilin的N末端片段（aa 33-214）的重组蛋白。
表位：N末端片段（aa 33-214）

Other Notes

浓度：请参考特定批次的数据表。

Physical form

形式：纯化
纯化的小鼠单克隆IgG1κ，溶于含0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
纯化蛋白G

Preparation Note

自接收之日起，在2-8°C下可稳定保存1年。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Maximilian Binter et al.
PloS one, 18(12), e0296124-e0296124 (2023-12-21)
The outflow pathway, especially trabecular meshwork (TM), plays an essential role in glaucoma, and the availability of TM cells is crucial for in vitro research. So far, the isolation of TM cells from mice has been anything but manageable due
Biting Zhou et al.
Frontiers in genetics, 13, 1019208-1019208 (2022-10-22)
MYOC is a common pathogenic gene for primary open-angle glaucoma and encodes the protein named myocilin. Multiple MYOC variations have been found, with different clinical significance. However, the pathogenesis of glaucoma induced by MYOC mutations has not been fully clarified.
Kate E Keller et al.
Experimental eye research, 171, 164-173 (2018-03-13)
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the
Sarah M Judge et al.
Cancer research, 80(9), 1861-1874 (2020-03-07)
Skeletal muscle wasting is a devastating consequence of cancer that contributes to increased complications and poor survival, but is not well understood at the molecular level. Herein, we investigated the role of Myocilin (Myoc), a skeletal muscle hypertrophy-promoting protein that
Philip Mzyk et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Although the extracellular matrix (ECM) in trabecular meshwork (TM) cells is known to be important in intraocular pressure (IOP) regulation, the molecular mechanisms involved in generating a glaucomatous environment in the TM are not completely understood. Recently we identified a

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