Anti-TrpV6 Antibody, clone 4A5.1

Transient receptor potential cation channel subfamily V member 6 (UniProt Q9H1D0; also known as Alu-binding protein with zinc finger domain, Calcium transport protein 1, Calcium transporter-like protein, CaT-L, CaT-like, CaT1, ECaC2, Epithelial apical membrane calcium transporter/channel CaT1, Epithelial calcium channel 2, TrpV6) is encoded by the TRPV6 (also known as ABP/ZF, CAT1, CATL, ECAC2, ZFAB) gene (Gene ID 55503) in human. TRPV6 belongs to the transient receptor potential (TRP) family of membrane proteins. The human TRPV6 is an 765-amino acid, six-transmembrane protein with both N- and C-termini exposed intracellularly. TRPV6 contains a pore-forming loop domain located between the fifth and sixth transmembrane region. Among all the TRPV family members, TRPV5 and TRPV6 are the two highly Ca2+-selective channels that play pivotal roles in the Ca2+-reabsorption in the kidney and intestine, respectively. TRPV6 is found in the apical brush-border membrane of the intestinal enterocytes where it regulates calcium entry into the cells. TRPV6 expression is also reported in human placenta, pancreas, and prostate gland. Vitamin D treatment of human colon cancer Caco-2 cells and duodenal explants results in upregulated TRPV6 mRNA levels.

Expected to react with both spliced isoforms of human TrpV6 reported by UniProt (Q9H1D0).

Epitope: C-terminal region.

GST-tagged recombinant human TrpV6 C-terminal fragment.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected TrpV6 in 10 µg of LNCaP prostate adenocarcinoma and K562 erythroleukemia cell lysates.
Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected TrpV6 in human pancreas, human small intestine, rat colon tissue, and mouse brain tissues.

Evaluated by Western Blotting in Y79 retinoblastoma cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TrpV6 in 10 µg of Y79 retinoblastoma cell lysate.

~70 kDa observed.

Unpurified

In culture supernatant without preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

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