Anti-VPS4A Antibody, clone 14B1.1

Vacuolar protein sorting-associated protein 4A (EC 3.6.4.6; UniProt Q9UN37; also known as hVPS4, Protein SKD2, SKD1-homolog, Vacuolar protein sorting factor 4A, Vacuolar sorting protein 4, VPS4A) is encoded by the VPS4A gene (also known as SKD1A, VPS4, VPS4-1) (Gene ID 27183) in human. Vps4 is a member of the ATPases associated with various cellular activities. The ESCRT (endosomal sorting complexes required for transport) machinery consists of five complexes (SCRT-0, ESCRT-I, ESCRT-II, ESCRT-III, and Vps4) that mediate multiple cellular membrane remodeling events, ranging from multivesicular bodies and exosomes formation to the final membrane abscission stage of cytokinesis, all of which converge in the scission of the narrow membrane neck that connects bud to parent membrane or daughter cell to daughter cell. Scission depends on the polymerization of soluble ESCRT-III monomers or dimers into tightly membrane-bound filaments. Vps4 is essential for ESCRT function in cells and it mediates the disassembly of ESCRT-III filaments to ensure a constant replenishing of the pool of soluble ESCRT-III monomers. The ESCRTs are also exploited by HIV-1 and many other enveloped viruses for their escape from the host cells. Vps4A is reported to facilitate the secretion of oncogenic miRNAs in exosomes and downregulation of Vps4A in hepatocellular carcinoma (HCC) is associated with tumor progression and metastasis.

Epitope: Near N-terminus

GST-tagged recombinant protein corresponding to human VPS4A sequence at the N-terminus.

Detect VPS4A using this Anti-VPS4A Antibody, clone 14B1.1 validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected VPS4A in 200 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected VPS4A in rat cerebral cortex tissue.

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VPS4A in 200 µg of Jurkat cell lysate.

~50 kDa observed. Uncharacterized band(s) may appear in some lysates.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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