Anti-ARNTL Antibody, clone 1C5

Aryl hydrocarbon receptor nuclear translocator-like protein 1 or alternatively, Basic-helix-loop-helix PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5 (bHLHe5), Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3, and encoded by the human gene ARNTL,BHLHE5, BMAL1, MOP3 or PASD3, is a protein belonging to the basic helix-loop-helix-PAS domain containing transcription factor family. ARNTL protein is important for the molecular mechanisms behind circadian rhythms as it interacts with a number of CLOCK (Circadian Locomotor Output Cycles Kaput) family members as well as CLOCK itself to promote transcription of a number of proteins of the circadian clock. ARNTL interacts with CRY and CRY2, PER, TIMELESS, and other clock related genes as well as non-CLOCK proteins such as HIF-1alpha (HIF1A) and SUMO3. ARNTL appears critical for proper function of rhythms, as only knockouts of ARNTL in mice generate mice that have upset biological clocks and a shorter lifespan. ARNTL is highly expressed in the adult brain as well as skeletal muscle and heart and ARNTL is a nuclear localized transcription activator. EMD-Millipore’s Anti-ARNTL mouse monoclonal antibody has been tested in western blot against recombinant transfected protein and in paraffin embedded immunohistochemistry on human spleen tissue sections.

Purified recombinant fragment of human ARNTL expressed in E. Coli.

Anti-ARNTL Antibody, clone 1C5 is a highly specific mouse monoclonal antibody, that targets ARNT-Like 1 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ARNTL in human spleen tissue.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Evaluated by Western Blotting in ARNTL-hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ARNTL-hIgGFc transfected HEK293 cell lysate.

~100 kDa observed. Uncharacterized bands may appear in some lysate(s).

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
ARNTL-hIgGFc transfected HEK293 cell lysate

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