Anti-LAR/PTPRF Antibody, clone N165/38

Receptor-linked protein-tyrosine phosphatases (RPTPs) are signaling proteins that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. Leukocyte common antigen receptor protein tyrosine phosphatase (LAR/PTPRF), a negative regulator of several receptor tyrosine kinases, is present in high concentrations in mature synapses and is considered to be important for the development and maintenance of excitatory synapses in hippocampal neurons. Disruption of LAR function is shown to result in loss of excitatory synapses, diminution in surface AMPA receptors, and impairment of dendritic targeting of the cadherin–beta-catenin complex. LAR is also present in neurons expressing TrkB where it participates in survival of neurons and the neurite outgrowth. Increased expression of LAR in insulin-responsive tissues has also been associated with obesity and insulin resistance.

Other Homologies: Mouse (97% sequence homology).

This antibody does not cross-react with PTPRD and PTPRS (Prof. J. Trimmer, University of California, Davis.).

Recombinant protein corresponding to human LAR/PTPRF.

Anti-LAR/PTPRF Antibody, clone N165/38 is a highly specific mouse monoclonal antibody & that targets LAR & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Evaluated by Western Blotting in rat brain membrane tissue.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected LAR/PTPRF in 10 µg of rat brain membrane tissue.

~78 kDa observed. The full length protein is 210 kDa; however, it runs lower in Western Blotting due to proteolysis into P-subunit containing transmembrane and intracellular domains (Prof. J. Trimmer, University of California, Davis).

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat brain membrane tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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